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Nrf2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1312-8

Nrf2 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Nrf2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Nrf2: 401-500/605

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 68 kDa

Conjugation

unconjugated

RRID

AB_3073268

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:100-1000

  • IHC-P

  • 1:100-500

  • IF-tissue

  • 1:100-500

Target

Function

The Nrf2 antioxidant response pathway is "the primary cellular defense against the cytotoxic effects of oxidative stress." Among other effects, NFE2L2 increases the expression of several antioxidant enzymes. Several drugs that stimulate the NFE2L2 pathway are being studied for treatment of diseases that are caused by oxidative stress, and dimethyl fumarate significantly reduced the progression of disability in multiple sclerosis. Under normal or unstressed conditions, Nrf2 is kept in the cytoplasm by a cluster of proteins that degrade it quickly. Under oxidative stress, Nrf2 is not degraded, but instead travels to the nucleus where it binds to a DNA promoter and initiates transcription of antioxidative genes and their proteins.

Background References

1. "Structure of the Keap1:Nrf2 interface provides mechanistic insight into Nrf2 signaling." Lo S.-C., Li X., Henzl M.T., Beamer L.J., Hannink M. EMBO J. 25:3605-3617(2006)

2. "Acetylation-deacetylation of the transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2) regulates its transcriptional activity and nucleocytoplasmic localization." Kawai Y., Garduno L., Theodore M., Yang J., Arinze I.J. J. Biol. Chem. 286:7629-7640(2011)

3. "Transformation of eEF1Bdelta into heat-shock response transcription factor by alternative splicing." Kaitsuka T., Tomizawa K., Matsushita M. EMBO Rep. 12:673-681(2011)

Sequence Similarity

Belongs to the bZIP family. CNC subfamily.

Tissue Specificity

Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.

Post-translational Modification

Ubiquitinated in the cytoplasm by the BCR(KEAP1) E3 ubiquitin ligase complex leading to its degradation. In response to oxidative stress, electrophile metabolites, such as sulforaphane, modify KEAP1, leading to inhibit activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and activity. In response to autophagy, the BCR(KEAP1) complex is inactivated (By similarity).; Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.; Acetylation at Lys-596 and Lys-599 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence.; Glycation impairs transcription factor activity by preventing heterodimerization with small Maf proteins. Deglycation by FN3K restores activity.

Subcellular Location

Cytoplasm, Nucleus

UNIPROT

SwissProt: Q16236 Human

SwissProt: Q60795 Mouse

Synonyms

erythroid derived 2 antibody

HEBP1 antibody

like 2 antibody

NF E2 related factor 2 antibody

NF-E2-related factor 2 antibody

NF2L2_HUMAN antibody

NFE2 related factor 2 antibody

NFE2-related factor 2 antibody

Nfe2l2 antibody

Nrf 2 antibody

Expand

Images

  • Hep-G2 (H) cells were treated with or without MG-132 (10 μM) for 10h, 25 μg total protein per lane of cell lysates (see on figure) probed with Nrf2 polyclonal antibody, unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

    Hep-G2 (H) cells were treated with or without MG-132 (10 μM) for 10h, 25 μg total protein per lane of cell lysates (see on figure) probed with Nrf2 polyclonal antibody, unconjugated (R1312-8) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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