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OGG1 Recombinant Rabbit Monoclonal Antibody [JA18-13]

Usd: 385

Specification

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Catalog# ET1704-79

OGG1 Recombinant Rabbit Monoclonal Antibody [JA18-13]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

OGG1 Recombinant Rabbit Monoclonal Antibody [JA18-13]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human OGG1 aa 296-345 / 345.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 39 kDa

Positive Control

Hep G2 (Human hepatocellular carcinoma cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, 293T (Human embryonic kidney cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, Daudi (Human Burkitt's lymphoma cell) cell lysate, human kidney tissue.

Conjugation

unconjugated

Clone Number

JA18-13

RRID

AB_3070522

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:50-1:200

Target

Function

DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion. The OGG1 gene encodes 8 isoforms (OGG1A-C, OGG2A-E) which result from alternative splicing of a single messenger RNA. The OGG1A splice variant is the most prevalent form and localizes to the nucleus, whereas the OGG2A splice variant is targeted to the mitochondria.

Background References

1. Zhao F et al. OGG1 in the Kidney: Beyond Base Excision Repair. Oxid Med Cell Longev. 2022 Dec

2. Ma X et al. OGG1 in Lung-More than Base Excision Repair. Antioxidants (Basel). 2022 May

Sequence Similarity

Belongs to the type-1 OGG1 family.

Tissue Specificity

Ubiquitous.

Subcellular Location

Nucleus, nucleoplasm, Nucleus speckle, Nucleus matrix; Mitochondrion.

UNIPROT

SwissProt: O15527 Human

Synonyms

8 hydroxyguanine DNA glycosylase antibody

8 oxoguanine DNA glycosylase 1 antibody

8 oxoguanine DNA glycosylase antibody

AP lyase antibody

DNA (apurinic or apyrimidinic site) lyase antibody

DNA apurinic or apyrimidinic site lyase antibody

DNA lyase antibody

DNA-(apurinic or apyrimidinic site) lyase antibody

HMMH antibody

HOGG 1 antibody

Expand

Images

  • Western blot analysis of OGG1 on different lysates with Rabbit anti-OGG1 antibody (<a href="/products/ET1704-79" style="font-weight: bold;text-decoration: underline;">ET1704-79</a>) at 1/5,000 dilution.<br /><br />Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate<br />Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate<br />Lane 3: 293T (Human embryonic kidney cell) cell lysate<br />Lane 4: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 5: Raji (Human Burkitt's lymphoma cell) cell lysate<br />Lane 6: Daudi (Human Burkitt's lymphoma cell) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 1 minute 2 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1704-79" style="font-weight: bold;text-decoration: underline;">ET1704-79</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 39 kDa<br />Observed band size: 35 kDa

    Western blot analysis of OGG1 on different lysates with Rabbit anti-OGG1 antibody (ET1704-79) at 1/5,000 dilution.

    Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
    Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate
    Lane 3: 293T (Human embryonic kidney cell) cell lysate
    Lane 4: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 5: Raji (Human Burkitt's lymphoma cell) cell lysate
    Lane 6: Daudi (Human Burkitt's lymphoma cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 1 minute 2 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1704-79, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 39 kDa
    Observed band size: 35 kDa

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-OGG1 antibody (<a href="/products/ET1704-79" style="font-weight: bold;text-decoration: underline;">ET1704-79</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-79" style="font-weight: bold;text-decoration: underline;">ET1704-79</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-OGG1 antibody (ET1704-79) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-79) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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