OGG1 Recombinant Rabbit Monoclonal Antibody [JA18-13]
Catalog# ET1704-79
OGG1 Recombinant Rabbit Monoclonal Antibody [JA18-13]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
OGG1 Recombinant Rabbit Monoclonal Antibody [JA18-13]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human OGG1 aa 296-345 / 345.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
39 kDa
Positive Control
Human kidney tissue, Daudi cell lysates.
Conjugation
unconjugated
Clone Number
JA18-13
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500
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IHC-P
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1:50-1:200
Target
Function
DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion. The OGG1 gene encodes 8 isoforms (OGG1A-C, OGG2A-E) which result from alternative splicing of a single messenger RNA. The OGG1A splice variant is the most prevalent form and localizes to the nucleus, whereas the OGG2A splice variant is targeted to the mitochondria.
Background References
1. Yang C et al. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A.Nucleic Acids Res 45:739-748 (2017).
2. Sassa A et al. Xeroderma Pigmentosum Group A Suppresses Mutagenesis Caused by Clustered Oxidative DNA Adducts in the Human Genome. PLoS One 10:e0142218 (2015).
Sequence Similarity
Belongs to the type-1 OGG1 family.
Tissue Specificity
Ubiquitous.
Subcellular Location
Mitochondrion. Nucleus
UNIPROT
Synonyms
8 hydroxyguanine DNA glycosylase antibody
8 oxoguanine DNA glycosylase 1 antibody
8 oxoguanine DNA glycosylase antibody
AP lyase antibody
DNA (apurinic or apyrimidinic site) lyase antibody
DNA apurinic or apyrimidinic site lyase antibody
DNA lyase antibody
DNA-(apurinic or apyrimidinic site) lyase antibody
HMMH antibody
HOGG 1 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-OGG1 antibody (ET1704-79) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-79) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of OGG1 on Daudi cell lysates with Rabbit anti-OGG1 antibody (ET1704-79) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-79) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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