OLFM4 Recombinant Rabbit Monoclonal Antibody [PSH01-36] - BSA and Azide free
Catalog# HA750714
OLFM4 Recombinant Rabbit Monoclonal Antibody [PSH01-36] - BSA and Azide free
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
OLFM4 Recombinant Rabbit Monoclonal Antibody [PSH01-36] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human OLFM4 aa 1-510 / 510.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 57 kDa
Positive Control
Human liver tissue.
Conjugation
unconjugated
Clone Number
PSH01-36
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Olfactomedin 4 is a protein that in humans is encoded by the OLFM4 gene. This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflamed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion.
Background References
1. Gong F et al. OLFM4 Regulates Lung Epithelial Cell Function in Sepsis-Associated ARDS/ALI via LDHA-Mediated NF-κB Signaling. J Inflamm Res. 2021 Dec
2. Chen Z et al. OLFM4 deficiency delays the progression of colitis to colorectal cancer by abrogating PMN-MDSCs recruitment. Oncogene. 2022 May
Subcellular Location
Secreted, extracellular space, Mitochondrion.
UNIPROT
Synonyms
Antiapoptotic protein GW112 antibody
bA209J19.1 antibody
G-CSF-stimulated clone 1 protein antibody
GC1 antibody
GW112 antibody
hGC 1 antibody
hGC-1 antibody
hOLfD antibody
KIAA4294 antibody
olfactomedin 4 antibody
ExpandImages
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This data was developed using HA721670, the same antibody clone in a different buffer formulation.
Western blot analysis of OLFM4 on different lysates with Rabbit anti-OLFM4 antibody (HA721670) at 1/1,000 dilution.
Lane 1: OLFM4 recombinant protein
Lane 2: OLFM4 recombinant protein with immunising peptide
Lysates/proteins at 50 ng/Lane.
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721670) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721670, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-OLFM4 antibody (HA721670) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721670) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
OLFM4 Recombinant Rabbit Monoclonal Antibody [PSH01-36]
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
OLFM4 Recombinant Rabbit Monoclonal Antibody [PSH01-37] - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
