Optineurin Rabbit Polyclonal Antibody
Catalog# ER1914-40
Optineurin Rabbit Polyclonal Antibody
-
WB
-
IHC-P
-
IF-Tissue
-
Mouse
-
unconjugated
Overview
Product Name
Optineurin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from human Optineurin 341-440/577
Species Reactivity
Mouse
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
66 KDa
Positive Control
Present in acqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-2000
-
IHC-P
-
1:100-500
-
IF-tissue
-
1:100-500
Target
Function
Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death.
Background References
暂无
Subcellular Location
Cytoplasm > perinuclear region. Golgi apparatus.
UNIPROT
Synonyms
14.7K interacting protein antibody
Ag9 C5 antibody
ALS12 antibody
E3 14.7K interacting protein antibody
E3-14.7K-interacting protein antibody
FIP 2 antibody
FIP-2 antibody
FIP2 antibody
Glaucoma 1 open angle E (adult onset) antibody
Glaucoma 1 open angle E antibody
ExpandImages
-
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-Optineurin(ER1914-40)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66kD
Observed band size: 66kD -
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Optineurin) Polyclonal Antibody, Unconjugated (ER1914-40) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
-
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Optineurin) Polyclonal Antibody, Unconjugated (ER1914-40) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Optineurin Rabbit Polyclonal Antibody
Application: WB,FC
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway