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Osteopontin Recombinant Rabbit Monoclonal Antibody [PSH09-23] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751277

Osteopontin Recombinant Rabbit Monoclonal Antibody [PSH09-23] - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Osteopontin Recombinant Rabbit Monoclonal Antibody [PSH09-23] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Osteopontin aa 1-314.

Species Reactivity

Human

Validated Applications

IHC-Fr, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 35 kDa

Positive Control

Human kidney tissue, human bone tissue.

Conjugation

unconjugated

Clone Number

PSH09-23

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:200

  • IHC-P

  • 1:2,000

  • IF-Tissue

  • 1:200-1:500

Target

Function

Osteopontin (OPN), also known as bone /sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The murine ortholog is Spp1. Osteopontin is a SIBLING (glycoprotein) that was first identified in 1986 in osteoblasts. The prefix osteo- indicates that the protein is expressed in bone, although it is also expressed in other tissues. The suffix -pontin is derived from "pons," the Latin word for bridge, and signifies osteopontin's role as a linking protein. Osteopontin is an extracellular structural protein and therefore an organic component of bone.

Background References

1. Han H et al. Macrophage-derived Osteopontin (SPP1) Protects From Nonalcoholic Steatohepatitis. Gastroenterology. 2023 Jul

2. Shirakawa K et al. Osteopontin in Cardiovascular Diseases. Biomolecules. 2021 Jul

Subcellular Location

Secreted.

UNIPROT

SwissProt: P10451 Human

SwissProt: P10923 Mouse

SwissProt: P08721 Rat

Synonyms

BNSP antibody

Bone sialoprotein 1 antibody

Bone sialoprotein I antibody

BSP I antibody

BSPI antibody

Early T lymphocyte activation 1 antibody

ETA 1 antibody

ETA1 antibody

MGC110940 antibody

Nephropontin antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Osteopontin antibody (<a href="/products/HA751277" style="font-weight: bold;text-decoration: underline;">HA751277</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751277" style="font-weight: bold;text-decoration: underline;">HA751277</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Osteopontin antibody (HA751277) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751277) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded human bone tissue labeling Osteopontin with Rabbit anti-Osteopontin antibody (<a href="/products/HA751277" style="font-weight: bold;text-decoration: underline;">HA751277</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) overnight at 60℃ water bath. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751277" style="font-weight: bold;text-decoration: underline;">HA751277</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunofluorescence analysis of paraffin-embedded human bone tissue labeling Osteopontin with Rabbit anti-Osteopontin antibody (HA751277) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) overnight at 60℃ water bath. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751277) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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