HRP Conjugated P-Selectin / CD62P Recombinant Rabbit Monoclonal Antibody [PSH16-71] - Detector
Overview
Product Name
HRP Conjugated P-Selectin / CD62P Recombinant Rabbit Monoclonal Antibody [PSH16-71] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse P-Selectin / CD62P aa 42-709 (HA211140).
Species Reactivity
Human, Mouse, Rat
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human P-Selectin / CD62P protein (HA210931) or Recombinant Mouse P-Selectin / CD62P protein (HA211140).
Conjugation
HRP
Clone Number
PSH16-71
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-71] to P-Selectin / CD62P antibody (Detector) (HA723873) and Recombinant Human P-Selectin / CD62P protein (HA210931) or Recombinant Mouse P-Selectin / CD62P protein (HA211140) as the standard. The reference range value is 1,370-111,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
P-selectin is a type-1 transmembrane protein that in humans is encoded by the SELP gene. P-selectin functions as a cell adhesion molecule (CAM) on the surfaces of activated endothelial cells, which line the inner surface of blood vessels, and activated platelets. In unactivated endothelial cells, it is stored in granules called Weibel-Palade bodies. In unactivated platelets P-selectin is stored in α-granules. Other names for P-selectin include CD62P, Granule Membrane Protein 140 (GMP-140), and Platelet Activation-Dependent Granule to External Membrane Protein (PADGEM). It was first identified in endothelial cells in 1989.
Background References
1. Hegazy S et al. CD62P (P-selectin) expression as a platelet activation marker in patients with liver cirrhosis with and without cholestasis. Clin Exp Hepatol. 2021 Jun
2. Takada YK et al. The C-type lectin domain of CD62P (P-selectin) functions as an integrin ligand. Life Sci Alliance. 2023 Apr
Subcellular Location
Cell membrane.
Synonyms
Antigen CD62 antibody
CD62 antibody
CD62 antigen-like family member P antibody
CD62P antibody
GMP 140 antibody
GMP-140 antibody
GMP140 antibody
GMRP antibody
Granule membrane protein 140 antibody
Granulocyte membrane protein antibody
ExpandImages
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![Sandwich ELISA analysis of Human P-Selectin matched pair antibodies<br /><br />Capture: <a href="/products/HA723872" style="font-weight: bold;text-decoration: underline;">HA723872</a>, P-Selectin / CD62P Rabbit mAb [PSH16-70]<br />Detector: <a href="/products/HA723873" style="font-weight: bold;text-decoration: underline;">HA723873</a>, P-Selectin / CD62P Rabbit mAb [PSH16-71]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723872" style="font-weight: bold;text-decoration: underline;">HA723872</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human P-Selectin / CD62P protein (<a href="/products/HA210931" style="font-weight: bold;text-decoration: underline;">HA210931</a>) starting from 111,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723873" style="font-weight: bold;text-decoration: underline;">HA723873</a>, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723873H_1.jpg?v=20260604174721)
Sandwich ELISA analysis of Human P-Selectin matched pair antibodies
Capture: HA723872, P-Selectin / CD62P Rabbit mAb [PSH16-70]
Detector: HA723873, P-Selectin / CD62P Rabbit mAb [PSH16-71]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723872) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human P-Selectin / CD62P protein (HA210931) starting from 111,000 pg/ml to 0 pg/ml and detect antibody (HA723873, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
P-Selectin / CD62P Recombinant Rabbit Monoclonal Antibody [PSH19-03]
Application: WB,IHC-P,FC,IHC-Fr,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
P-Selectin / CD62P Recombinant Rabbit Monoclonal Antibody [PSH19-03] - BSA and Azide free
Application: WB,IHC-P,FC,IHC-Fr,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
P-Selectin / CD62P Recombinant Rabbit Monoclonal Antibody [PSH16-71] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
P-Selectin / CD62P Recombinant Rabbit Monoclonal Antibody [PSH16-70] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
