P2X3 Recombinant Rabbit Monoclonal Antibody [PSH28-01]
Catalog# HA724743
P2X3 Recombinant Rabbit Monoclonal Antibody [PSH28-01]
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WB
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IHC-Fr
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IHC-P
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IP
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Mouse
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Rat
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unconjugated
Overview
Product Name
P2X3 Recombinant Rabbit Monoclonal Antibody [PSH28-01]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse P2X3 aa 1-322.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-Fr, IHC-P, IP
Target Molecular Weight
Predicted band size: 44 kDa
Positive Control
Mouse dorsal root ganglion tissue lysate, Rat dorsal root ganglion tissue lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysates.
Conjugation
unconjugated
Clone Number
PSH28-01
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IHC-Fr | FC | IF-Cell | IP | |
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| mouse |
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| rat |
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| green monkey |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-Fr
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1:500
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IHC-P
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1:200-1:1,000
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IP
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1-2μg/sample
Target
Function
This gene encodes a member of the P2X purinergic receptor (purinoceptor) gene family which includes seven members (P2RX1 - P2RX7). P2X purinoceptors are a family of cation-permeable, ligand-gated ion channels that open in response to the binding of extracellular adenosine 5'-triphosphate (ATP). The encoded protein is a subunit of the trimeric P2X3 receptor ion channel which is expressed by sensory or autonomic neurons. A deficiency of the orthologous protein in mice is associated with reduced pain-related behavior and urinary bladder hyporeflexia.
Background References
1. Marucci G. et. al. Update on novel purinergic P2X3 and P2X2/3 receptor antagonists and their potential therapeutic applications. Expert Opin Ther Pat. 2019 Dec
2. Wang J. et. al. Druggable negative allosteric site of P2X3 receptors. Proc Natl Acad Sci U S A. 2018 May
Subcellular Location
Cell membrane.
Synonyms
ATP receptor antibody
MGC129956 antibody
P2rx3 antibody
P2RX3_HUMAN antibody
P2X purinoceptor 3 antibody
P2X receptor subunit 3 antibody
P2X3 antibody
Purinergic receptor antibody
Purinergic receptor P2X ligand gated ion channel 3 antibody
Purinergic receptor P2X3 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of P2X3 on different lysates with Rabbit anti-P2X3 antibody (HA724743) at 1/5,000 dilution.
Lane 1: Mouse dorsal root ganglion tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Rat dorsal root ganglion tissue lysate
Lane 4: Rat spleen tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 1 minute 40 seconds; ECL: K1802
Low expression: spleen (PMID: 15670583).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724743, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 44 kDa
Observed band size: 44-60 kDa -
Western blot analysis of P2X3 on Neuro-2a (Mouse brain neuroblastoma cell) cell lysate with Rabbit anti-P2X3 antibody (HA724743) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minute 40 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724743, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 44 kDa
Observed band size: 44-60 kDa -
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Dorsal root ganglion
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724743, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunofluorescence (IHC-Fr)
Species: Rat
Tissue: Dorsal root ganglion
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724743, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Dorsal root ganglion
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724743, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Spinal dorsal horn
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724743, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724743, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Low expression: spleen (PMID: 15670583). -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Dorsal root ganglion
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724743, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Spinal dorsal horn
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724743, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Immunoprecipitation (IP)
P2X3 was immunoprecipitated in 0.2 mg Neuro-2a (Mouse brain neuroblastoma cell) cell lysate with HA724743 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724743 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Neuro-2a cell lysate (input)
Lane 2: HA724743 IP in Neuro-2a cell lysate
Lane 3: Rabbit IgG instead of HA724743 in Neuro-2a cell lysate
Exposure time: 2 minutes 37 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA724743, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 44 kDa
Observed band size: 44-60 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"