PCB Recombinant Rabbit Monoclonal Antibody [JE40-24]
Catalog# HA721546
PCB Recombinant Rabbit Monoclonal Antibody [JE40-24]
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WB
-
IHC-P
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Mouse
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Rat
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Human
Predicted species support after-sales service
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unconjugated
Overview
Product Name
PCB Recombinant Rabbit Monoclonal Antibody [JE40-24]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human PCB aa 1050-1150.
Species Reactivity
Mouse, Rat (Predicted: Human)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 130 kDa
Positive Control
Mouse liver tissue lysate, rat liver tissue lysate, mouse liver tissue.
Conjugation
unconjugated
Clone Number
JE40-24
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
Pyruvate carboxylase (PC) encoded by the gene PC is an enzyme (EC 6.4.1.1) of the ligase class that catalyzes (depending on the species) the physiologically irreversible carboxylation of pyruvate to form oxaloacetate (OAA). It is an important anaplerotic reaction that creates oxaloacetate from pyruvate. PC contains a biotin prosthetic group and is typically localized to the mitochondria in eukaryotes with exceptions to some fungal species such as Aspergillus nidulans which have a cytosolic PC. PC requires magnesium and zinc or manganese for catalysis. PC from different organisms exhibit varying degrees of activation by acetyl-CoA, but vertebrate PC typically requires it for activity.
Background References
1. Kiesel VA et al. Pyruvate carboxylase and cancer progression. Cancer Metab. 2021 Apr
2. Schwörer S et al. Fibroblast pyruvate carboxylase is required for collagen production in the tumour microenvironment. Nat Metab. 2021 Nov
Subcellular Location
Mitochondrion matrix.
Synonyms
mitochondrial antibody
OTTHUMP00000235155 antibody
OTTHUMP00000235156 antibody
PC antibody
PCB antibody
Pcx antibody
PYC_HUMAN antibody
Pyruvate carboxylase antibody
Pyruvate carboxylase mitochondrial antibody
Pyruvic carboxylase antibody
Images
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Western blot analysis of PCB on different lysates with Rabbit anti-PCB antibody (HA721546) at 1/1,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 130 kDa
Observed band size: 130 kDa
Exposure time: 1 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721546) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-PCB antibody (HA721546) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721546) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"