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PCSK9 Recombinant Rabbit Monoclonal Antibody [JE30-37]

Usd: 385

Specification

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Catalog# HA722528

PCSK9 Recombinant Rabbit Monoclonal Antibody [JE30-37]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

PCSK9 Recombinant Rabbit Monoclonal Antibody [JE30-37]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human PCSK9 aa 550-590/692.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 74 kDa

Positive Control

Huh7 cell lysate, BxPC-3 cell lysate, HeLa cell lysate, A431 cell lysate, human colon cancer tissue, human kidney tissue, human pancreas tissue.

Conjugation

unconjugated

Clone Number

JE30-37

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • IP

  • 1-2μg/sample

Target

Function

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an enzyme encoded by the PCSK9 gene in humans on chromosome 1. It is the 9th member of the proprotein convertase family of proteins that activate other proteins. Similar genes (orthologs) are found across many species. As with many proteins, PCSK9 is inactive when first synthesized, because a section of peptide chains blocks their activity; proprotein convertases remove that section to activate the enzyme. The PCSK9 gene also contains one of 27 loci associated with increased risk of coronary artery disease.

Background References

1. Seidah NG et al. The Multifaceted Biology of PCSK9. Endocr Rev. 2022 May

2. Barale C et al. PCSK9 Biology and Its Role in Atherothrombosis. Int J Mol Sci. 2021 May

Subcellular Location

Cytoplasm, Secreted, Endosome, Lysosome, Cell surface, Endoplasmic reticulum, Golgi apparatus.

UNIPROT

SwissProt: Q8NBP7 Human

Synonyms

Convertase subtilisin/kexin type 9 preproprotein antibody

FH3 antibody

HCHOLA3 antibody

Hypercholesterolemia autosomal dominant 3 antibody

LDLCQ1 antibody

NARC 1 antibody

NARC-1 antibody

NARC1 antibody

Neural apoptosis regulated convertase 1 antibody

Neural apoptosis-regulated convertase 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of PCSK9 on different lysates with Rabbit anti-PCSK9 antibody (<a href="/products/HA722528" style="font-weight: bold;text-decoration: underline;">HA722528</a>) at 1/1,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate (negative)<br />Lane 2: Huh7 cell lysate<br />Lane 3: BxPC-3 cell lysate<br />Lane 4: HeLa cell lysate<br />Lane 5: A431 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 60/74 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722528" style="font-weight: bold;text-decoration: underline;">HA722528</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of PCSK9 on different lysates with Rabbit anti-PCSK9 antibody (HA722528) at 1/1,000 dilution.

    Lane 1: SH-SY5Y cell lysate (negative)
    Lane 2: Huh7 cell lysate
    Lane 3: BxPC-3 cell lysate
    Lane 4: HeLa cell lysate
    Lane 5: A431 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 60/74 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722528) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PCSK9 antibody (<a href="/products/HA722528" style="font-weight: bold;text-decoration: underline;">HA722528</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722528" style="font-weight: bold;text-decoration: underline;">HA722528</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PCSK9 antibody (HA722528) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722528) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PCSK9 antibody (<a href="/products/HA722528" style="font-weight: bold;text-decoration: underline;">HA722528</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722528" style="font-weight: bold;text-decoration: underline;">HA722528</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PCSK9 antibody (HA722528) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722528) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PCSK9 antibody (<a href="/products/HA722528" style="font-weight: bold;text-decoration: underline;">HA722528</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722528" style="font-weight: bold;text-decoration: underline;">HA722528</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PCSK9 antibody (HA722528) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722528) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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