PDCD6 Mouse Monoclonal Antibody [A6A7]
Catalog# HA600092
PDCD6 Mouse Monoclonal Antibody [A6A7]
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WB
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IF-Cell
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FC
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Human
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unconjugated
Overview
Product Name
PDCD6 Mouse Monoclonal Antibody [A6A7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human PDCD6 aa 1-191 / 191.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 22 kDa
Positive Control
K-562 cell lysate, HepG2 cell lysate, 293T cell lysate, HeLa cell lysate, HeLa.
Conjugation
unconjugated
Clone Number
A6A7
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:100
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FC
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1:500-1:1,000
Target
Function
Programmed cell death protein 6 is a protein that in humans is encoded by the PDCD6 gene. This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5.
Background References
1. Wang X. et. al. PDCD6 cooperates with C-Raf to facilitate colorectal cancer progression via Raf/MEK/ERK activation. J Exp Clin Cancer Res. 2020 Aug
2. Gong Y. et. al. miR-9875 functions in antiviral immunity by targeting PDCD6 in mud crab (Scylla paramamosain). Virulence. 2020 Dec
Subcellular Location
Endosome, Nucleus, Endoplasmic reticulum membrane, Cytoplasm, COPII-coated vesicle membrane.
UNIPROT
Synonyms
AIP1 antibody
ALG 2 antibody
ALG-2-interacting protein 1 antibody
ALG2 antibody
ALIX antibody
Apoptosis linked gene 2 antibody
Apoptosis linked gene 2 protein antibody
Apoptosis-linked gene 2 protein antibody
FLJ42309 antibody
FLJ46208 antibody
ExpandImages
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Western blot analysis of PDCD6 on different lysates with Mouse anti-PDCD6 antibody (HA600092) at 1/2,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: 293T cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600092) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling PDCD6 with Mouse anti-PDCD6 antibody (HA600092) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PDCD6 antibody (HA600092) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HeLa cells labeling PDCD6.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA600092, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
PDCD6 Recombinant Mouse Monoclonal Antibody [A6A7-R]
Application: WB,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway
PDCD6 Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Rat
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PDCD6 Recombinant Mouse Monoclonal Antibody [A6A7-R] - BSA and Azide free
Application: WB,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated