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PDE10A Recombinant Rabbit Monoclonal Antibody [PSH03-61] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750895

PDE10A Recombinant Rabbit Monoclonal Antibody [PSH03-61] - BSA and Azide free

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

PDE10A Recombinant Rabbit Monoclonal Antibody [PSH03-61] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human PDE10A aa 400-779 / 779.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 88 kDa

Positive Control

Human brain tissue lysate, mouse brain tissue lysate, mouse testis tissue lysate, rat brain tissue lysate, mouse brain tissue, rat brain tissue, SH-SY5Y.

Conjugation

unconjugated

Clone Number

PSH03-61

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase family. It plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP to the corresponding nucleoside 5' monophosphate, but has higher affinity for cAMP, and is more efficient with cAMP as substrate. Alternatively spliced transcript variants have been described for this gene. Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition.

Background References

1. Chen S et al. PDE10A Inactivation Prevents Doxorubicin-Induced Cardiotoxicity and Tumor Growth. Circ Res. 2023 Jul

2. Menniti FS et al. PDE10A Inhibitors-Clinical Failure or Window Into Antipsychotic Drug Action? Front Neurosci. 2021 Jan

Subcellular Location

Cytoplasm, cytosol.

UNIPROT

SwissProt: Q9Y233 Human

SwissProt: Q8CA95 Mouse

SwissProt: Q9QYJ6 Rat

Synonyms

5''-cyclic phosphodiesterase 10A antibody

cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody

cAMP and cAMP-inhibited cGMP 3'' antibody

FLJ11894 antibody

HSPDE 10A antibody

HSPDE10A antibody

PDE 10A antibody

PDE10_HUMAN antibody

Pde10a antibody

Phosphodiesterase 10A antibody

Expand

Images

  • This data was developed using <a href="/products/HA722020" style="font-weight: bold;text-decoration: underline;">HA722020</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of PDE10A on different lysates with Rabbit anti-PDE10A antibody (<a href="/products/HA722020" style="font-weight: bold;text-decoration: underline;">HA722020</a>) at 1/2,000 dilution.<br /><br />Lane 1: Human brain tissue lysate<br />Lane 2: Mouse brain tissue lysate<br />Lane 3: Mouse testis tissue lysate<br />Lane 4: Rat brain tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br />Exposure time: 24 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA722020" style="font-weight: bold;text-decoration: underline;">HA722020</a>, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 88 kDa<br />Observed band size: 88 kDa

    This data was developed using HA722020, the same antibody clone in a different buffer formulation.
    Western blot analysis of PDE10A on different lysates with Rabbit anti-PDE10A antibody (HA722020) at 1/2,000 dilution.

    Lane 1: Human brain tissue lysate
    Lane 2: Mouse brain tissue lysate
    Lane 3: Mouse testis tissue lysate
    Lane 4: Rat brain tissue lysate

    Lysates/proteins at 40 µg/Lane.
    Exposure time: 24 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA722020, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 88 kDa
    Observed band size: 88 kDa

  • This data was developed using <a href="/products/HA722020" style="font-weight: bold;text-decoration: underline;">HA722020</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PDE10A antibody (<a href="/products/HA722020" style="font-weight: bold;text-decoration: underline;">HA722020</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722020" style="font-weight: bold;text-decoration: underline;">HA722020</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722020, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PDE10A antibody (HA722020) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722020) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722020" style="font-weight: bold;text-decoration: underline;">HA722020</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PDE10A antibody (<a href="/products/HA722020" style="font-weight: bold;text-decoration: underline;">HA722020</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722020" style="font-weight: bold;text-decoration: underline;">HA722020</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722020, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PDE10A antibody (HA722020) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722020) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722020" style="font-weight: bold;text-decoration: underline;">HA722020</a>, the same antibody clone in a different buffer formulation.<br />Flow cytometric analysis of SH-SY5Y cells labeling PDE10A.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722020" style="font-weight: bold;text-decoration: underline;">HA722020</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    This data was developed using HA722020, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of SH-SY5Y cells labeling PDE10A.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722020, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

PDE10A Recombinant Rabbit Monoclonal Antibody [PSH03-61]

Application: WB,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated