PDIA2 Recombinant Rabbit Monoclonal Antibody [JF97-08]
Catalog# ET1702-70
PDIA2 Recombinant Rabbit Monoclonal Antibody [JF97-08]
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
PDIA2 Recombinant Rabbit Monoclonal Antibody [JF97-08]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human PDIA2 aa 405-450.
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 57 kDa
Positive Control
HEK-293 cell lysate, NIH/3T3 cell lysate, Human liver tissue lysate.
Conjugation
unconjugated
Clone Number
JF97-08
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000-1:2,000
Target
Function
Oxidoreductase-protein disulfide isomerase (PDI) is a homodimer consisting of subunits that catalyzes thiol-disulfide exchange, mediates folding of newly synthesized proteins and functions as a molecular chaperone. PDI localizes to the lumen of the endoplasmic reticulum (ER), where in conjunction with folding-helper proteins, such as immunoglobulin heavy chain binding protein (BiP), mediates tertiary and quaternary protein-processing. Cell surface PDI induces sulfhydryl-mediated conformational changes in integrin-mediated adhesion receptor-ligand interactions, thereby regulating integrin responses and cell adhesion. Additionally, PDI functions as a subunit of two more complex enzyme systems: the prolyl-4-hydroxylase and the triacylglycerol transfer proteins.
Background References
1. Obser T et al. Identification and characterization of the elusive mutation causing the historical von Willebrand Disease type IIC Miami. J Thromb Haemost 14:1725-35 (2016).
2. Koumangoye R & Delpire E The Ste20 kinases SPAK and OSR1 travel between cells through exosomes. Am J Physiol Cell Physiol 311:C43-53 (2016).
Sequence Similarity
Belongs to the protein disulfide isomerase family.
Subcellular Location
Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome, Cell membrane.
Synonyms
Pancreas specific protein disulfide isomerase antibody
Pancreas-specific protein disulfide isomerase antibody
Pancreatic protein disulfide isomerase antibody
PDA2 antibody
PDI antibody
PDIA2 antibody
PDIA2_HUMAN antibody
PDIp antibody
PDIR antibody
Protein disulfide isomerase A2 antibody
ExpandImages
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Western blot analysis of PDIA2 on different lysates with Rabbit anti-PDIA2 antibody (ET1702-70) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: Human liver tissue lysate (40 µg/Lane)
Predicted band size: 92 kDa
Observed band size: 92 kDa
Exposure time: Lane 1: 1 minute 18 seconds; Lane 2-3: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-70) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"