PEDF Recombinant Rabbit Monoclonal Antibody [PSH28-40] - BSA and Azide free
Catalog# HA752117
PEDF Recombinant Rabbit Monoclonal Antibody [PSH28-40] - BSA and Azide free
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WB
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FC(Intra)
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IP
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Human
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Mouse
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Rat
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Pig
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unconjugated
Overview
Product Name
PEDF Recombinant Rabbit Monoclonal Antibody [PSH28-40] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human PEDF aa 1-418.
Species Reactivity
Human, Mouse, Rat, Pig
Validated Applications
WB, FC(Intra), IP
Target Molecular Weight
Predicted band size: 46 kDa
Positive Control
K-562 (Human chronic myelogenous leukemia cell) cell lysate, Y79 (Human retinoblastoma cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysates, NIH/3T3 (Mouse fibroblast) cell lysate, 3T3-L1 (Mouse embryonic fibroblast) cell lysate, C2C12 (Mouse myoblast) cell lysate, NRK (Rat kidney cell) cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Mouse stomach tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, Human serum lysate, Mouse plasma lysate, Rat plasma lysate, Pig liver tissue lysate, Pig brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH28-40
Product Features
Form
Liquid
Concentration
0.5 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Pigment epithelium-derived factor (PEDF) also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functions. Found in vertebrates, this 50 kDa protein is being researched as a therapeutic candidate for treatment of such conditions as choroidal neovascularization, heart disease, and cancer.[5] In humans, pigment epithelium-derived factor is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. Endothelial cell migration is inhibited by PEDF. PEDF suppresses retinal neovascularization and endothelial cell proliferation. The antiangiogenic residues 24-57 were shown to be sufficient at inhibiting angiogenesis. PEDF is also responsible for apoptosis of endothelial cells either through the p38 MAPK pathway or through the FAS/FASL pathway Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. The antitumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF was shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. VEGF expression by cancer cells is inhibited by PEDF. PEDF also displays neurotrophic functions. Retinoblastoma cells differentiate into neurons due to the presence of PEDF. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.
Background References
1. Carpino G et al. Thrombospondin 1 and 2 along with PEDF inhibit angiogenesis and promote lymphangiogenesis in intrahepatic cholangiocarcinoma. J Hepatol. 2021 Dec
2. Ma B et al. Pigment epithelium-derived factor (PEDF) plays anti-inflammatory roles in the pathogenesis of dry eye disease. Ocul Surf. 2021 Apr
Subcellular Location
Secreted, Melanosome.
Synonyms
Cell proliferation-inducing gene 35 protein antibody
EPC 1 antibody
EPC-1 antibody
EPC1 antibody
OI12 antibody
OI6 antibody
PEDF antibody
PEDF_HUMAN antibody
PIG 35 antibody
PIG35 antibody
ExpandImages
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☑ Relative expression (RE)
This data was developed using HA724781, the same antibody clone in a different buffer formulation.
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA724781) at 1/2,500 dilution.
Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 2: Y79 (Human retinoblastoma cell) cell lysate
Lane 3: MCF7 (Human breast cancer cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 2 minutes 6 seconds; ECL: K1801
MCF7 is a negative control (PMID: 16740777).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 46 kDa
Observed band size: 50 kDa -
This data was developed using HA724781, the same antibody clone in a different buffer formulation.
Western blot analysis of PEDF on Hep G2 (Human hepatocellular carcinoma cell) cell lysate with Rabbit anti-PEDF antibody (HA724781) at 1/2,500 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 46 kDa
Observed band size: 50 kDa -
This data was developed using HA724781, the same antibody clone in a different buffer formulation.
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA724781) at 1/2,500 dilution.
Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 2: 3T3-L1 (Mouse embryonic fibroblast) cell lysate
Lane 3: C2C12 (Mouse myoblast) cell lysate
Lane 4: NRK (Rat kidney cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 18 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 46 kDa
Observed band size: 50 kDa -
This data was developed using HA724781, the same antibody clone in a different buffer formulation.
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA724781) at 1/2,500 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Mouse stomach tissue lysate
Lane 4: Rat liver tissue lysate
Lane 5: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minute 2 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 46 kDa
Observed band size: 50 kDa -
This data was developed using HA724781, the same antibody clone in a different buffer formulation.
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA724781) at 1/2,500 dilution.
Lane 1: Human serum lysate
Lane 2: Mouse plasma lysate
Lane 3: Rat plasma lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 18 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 46 kDa
Observed band size: 50 kDa -
This data was developed using HA724781, the same antibody clone in a different buffer formulation.
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA724781) at 1/2,500 dilution.
Lane 1: Pig liver tissue lysate
Lane 2: Pig brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minute 2 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 46 kDa
Observed band size: 50 kDa -
This data was developed using HA724781, the same antibody clone in a different buffer formulation.
Application: Flow Cytometry (Intra)
Species: Mouse
Sample: NIH/3T3 (Mouse fibroblasts)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724781 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. -
This data was developed using HA724781, the same antibody clone in a different buffer formulation.
Immunoprecipitation (IP)
PEDF was immunoprecipitated in 0.2 mg Hep G2 (Human hepatocellular carcinoma cell) cell lysate with HA724781 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724781 at 1/2,500 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Hep G2 cell lysate (input)
Lane 2: HA724781 IP in Hep G2 cell lysate
Lane 3: Rabbit IgG instead of HA724781 in Hep G2 cell lysate
Exposure time: 12 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 46 kDa
Observed band size: 50 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
PEDF Recombinant Rabbit Monoclonal Antibody [PSH28-40]
Application: WB,FC(Intra),IP
Reactivity: Human,Mouse,Rat,Pig
Conjugate: unconjugated
