Skip to content

PERK Rabbit Polyclonal Antibody

Usd:

Bulk Order Quote | Customization Request

Catalog# ER64553

PERK Rabbit Polyclonal Antibody

Application

  • IF-Cell

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PERK Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

The antiserum was produced against synthesized peptide derived from human EIF2AK3. AA range:947-996

Species Reactivity

Human, Mouse, Rat

Validated Applications

IF-Cell, WB, IHC-P, IF-Tissue

Molecular Weight

125 kDa

Positive Control

MCF-7 cell, human colon carcinoma, rat-spleen.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:100-1:300

  • IF-Tissue

  • 1:50-1:200

Target

Function

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Background References

暂无

Subcellular Location

Endoplasmic reticulum membrane.

UNIPROT

SwissProt: Q9NZJ5 Human

Synonyms

DKFZp781H1925 antibody

E2AK3_HUMAN antibody

EC 2.7.11.1 antibody

Eif2ak3 antibody

Eukaryotic translation initiation factor 2 alpha kinase 3 antibody

Eukaryotic translation initiation factor 2-alpha kinase 3 antibody

Heme regulated EIF2 alpha kinase antibody

HRI antibody

HsPEK antibody

Pancreatic eIF2 alpha kinase antibody

Expand

Images

  • Western blot analysis of lysates from MCF-7 cells, using PEK/PERK Antibody. The lane on the right is blocked with the synthesized peptide.

    Western blot analysis of lysates from MCF-7 cells, using PEK/PERK Antibody. The lane on the right is blocked with the synthesized peptide.

  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PEK/PERK Antibody. The picture on the right is blocked with the synthesized peptide.

    Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PEK/PERK Antibody. The picture on the right is blocked with the synthesized peptide.

  • Immunofluorescence analysis of rat-spleen tissue. 1,PERK Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

    Immunofluorescence analysis of rat-spleen tissue. 1,PERK Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

Products with the same target and pathway

metafield image

PERK Rabbit Polyclonal Antibody

Application: WB

Reactivity: Human

Conjugate: unconjugated

metafield image

PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81]

Application: WB

Reactivity: Human

Conjugate: unconjugated