Skip to content

PI 3 Kinase p85 alpha Rabbit Polyclonal Antibody

Usd: 315

Specification

Bulk Order Quote | Customization Request

Catalog# R1312-6

PI 3 Kinase p85 alpha Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PI 3 Kinase p85 alpha Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within C-terminal human PI3-kinase p85 subunit alpha.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 84 kDa

Positive Control

Jurkat, PC12, NIH/3T3, HepG2, human liver tissue, human kidney tissue, mouse colon tissue, Hela.

Conjugation

unconjugated

RRID

AB_3073267

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100-1:200

  • IHC

  • 1:200-1:500

Target

Function

Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in humans is encoded by the PIK3R1 gene. Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. Mutations in PIK3R1 are implicated in cases of breast cancer.Mutations in PIK3R1 are associated to SHORT syndrome.

Background References

1. "Natural variants of human p85 alpha phosphoinositide 3-kinase in severe insulin resistance: a novel variant with impaired insulin-stimulated lipid kinase activity." Baynes K.C.R., Beeton C.A., Panayotou G., Stein R., Soos M., Hansen T., Simpson H., O\'Rahilly S., Shepherd P.R., Whitehead J.P. Diabetologia 43:321-331(2000)

2. "The structure of a human p110alpha/p85alpha complex elucidates the effects of oncogenic PI3Kalpha mutations." Huang C.-H., Mandelker D., Schmidt-Kittler O., Samuels Y., Velculescu V.E., Kinzler K.W., Vogelstein B., Gabelli S.B., Amzel L.M. Science 318:1744-1748(2007)

Sequence Similarity

Belongs to the PI3K p85 subunit family.

Tissue Specificity

Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

Post-translational Modification

Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.; Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.

Subcellular Location

Cytoplasm, nucleus

UNIPROT

SwissProt: P27986 Human

SwissProt: P26450 Mouse

SwissProt: Q63787 Rat

Synonyms

GRB1 antibody

p85 alpha antibody

p85 antibody

P85A_HUMAN antibody

Phosphatidylinositol 3 kinase associated p 85 alpha antibody

Phosphatidylinositol 3 kinase regulatory 1 antibody

Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody

Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody

Phosphatidylinositol 3-kinase regulatory subunit alpha antibody

Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody

Expand

Images

  • Western blot analysis on cell lysates using anti- PI3-kinase p85 subunit alpha rabbit polyclonal antibodies.

    Western blot analysis on cell lysates using anti- PI3-kinase p85 subunit alpha rabbit polyclonal antibodies.

  • ICC staining PI3-kinase p85 subunit alpha in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining PI3-kinase p85 subunit alpha in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunocytochemistry analysis of HepG2 cells labeling PI 3 Kinase p85 alpha with Rabbit anti-PI 3 Kinase p85 alpha antibody (<a href="/products/R1312-6" style="font-weight: bold;text-decoration: underline;">R1312-6</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PI 3 Kinase p85 alpha antibody (<a href="/products/R1312-6" style="font-weight: bold;text-decoration: underline;">R1312-6</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of HepG2 cells labeling PI 3 Kinase p85 alpha with Rabbit anti-PI 3 Kinase p85 alpha antibody (R1312-6) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PI 3 Kinase p85 alpha antibody (R1312-6) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PI 3 Kinase p85 alpha antibody (<a href="/products/R1312-6" style="font-weight: bold;text-decoration: underline;">R1312-6</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/R1312-6" style="font-weight: bold;text-decoration: underline;">R1312-6</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PI 3 Kinase p85 alpha antibody (R1312-6) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1312-6) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PI 3 Kinase p85 alpha antibody (<a href="/products/R1312-6" style="font-weight: bold;text-decoration: underline;">R1312-6</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/R1312-6" style="font-weight: bold;text-decoration: underline;">R1312-6</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PI 3 Kinase p85 alpha antibody (R1312-6) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1312-6) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PI 3 Kinase p85 alpha antibody (<a href="/products/R1312-6" style="font-weight: bold;text-decoration: underline;">R1312-6</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/R1312-6" style="font-weight: bold;text-decoration: underline;">R1312-6</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PI 3 Kinase p85 alpha antibody (R1312-6) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1312-6) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of Hela cells labeling PI 3 Kinase p85 alpha.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/R1312-6" style="font-weight: bold;text-decoration: underline;">R1312-6</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Hela cells labeling PI 3 Kinase p85 alpha.

    Cells were fixed and permeabilized. Then stained with the primary antibody (R1312-6, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

metafield image

PI 3 Kinase p85 alpha Mouse Monoclonal Antibody [A3-D0]

Application: WB,IF-Cell

Reactivity: Human

Conjugate: unconjugated

metafield image

PI 3 Kinase p85 alpha Recombinant Rabbit Monoclonal Antibody [SU04-07]

Application: WB,IF-Cell,IF-Tissue,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

PI 3 Kinase p85 alpha Recombinant Rabbit Monoclonal Antibody [JE04-11]

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

PI 3 Kinase p85 alpha Mouse Monoclonal Antibody [L3-D6]

Application: WB,IHC-P,IF-Cell,IF-Tissue

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

PI 3 Kinase p85 alpha Recombinant Mouse Monoclonal Antibody [A3-D0-R]

Application: WB,IF-Cell

Reactivity: Human

Conjugate: unconjugated

Products with the same target and pathway

metafield image

PI 3 Kinase p85 alpha Mouse Monoclonal Antibody [A3-D0]

Application: WB,IF-Cell

Reactivity: Human

Conjugate: unconjugated

metafield image

PI 3 Kinase p85 alpha Recombinant Rabbit Monoclonal Antibody [SU04-07]

Application: WB,IF-Cell,IF-Tissue,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

PI 3 Kinase p85 alpha Mouse Monoclonal Antibody [L3-D6]

Application: WB,IHC-P,IF-Cell,IF-Tissue

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated