PICALM Recombinant Rabbit Monoclonal Antibody [PSH01-64] - BSA and Azide free
Catalog# HA750740
PICALM Recombinant Rabbit Monoclonal Antibody [PSH01-64] - BSA and Azide free
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WB
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Human
-
Mouse
-
Rat
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unconjugated
Overview
Product Name
PICALM Recombinant Rabbit Monoclonal Antibody [PSH01-64] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human PICALM aa 1-450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 71 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, PC-3M cell lysate, HCT 116 cell lysate, 293T cell lysate, Neuro-2a cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
PSH01-64
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).
Background References
1. Moshkanbaryans L., Xue J., Wark J.R., Robinson P.J., Graham M.E. A Novel Sequence in AP180 and CALM Promotes Efficient Clathrin Binding and Assembly. PLoS ONE 11:E0162050-E0162050 (2016)
2. Sahlender D.A., Kozik P., Miller S.E., Peden A.A., Robinson M.S. Uncoupling the functions of CALM in VAMP sorting and clathrin-coated pit formation. PLoS ONE 8:E64514-E64514 (2013)
Subcellular Location
Cell membrane, Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus
Synonyms
CALM antibody
Clathrin assembly lymphoid myeloid leukemia antibody
Clathrin assembly lymphoid myeloid leukemia protein antibody
CLTH antibody
LAP antibody
Phosphatidylinositol-binding clathrin assembly protein antibody
PICAL_HUMAN antibody
PICALM antibody
Images
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Western blot analysis of PICALM on different lysates with Rabbit anti-PICALM antibody (HA750740) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: PC-3M cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: 293T cell lysate
Lane 6: Neuro-2a cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 65/71 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750740) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
PICALM Recombinant Rabbit Monoclonal Antibody [PSH01-63]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
PICALM Recombinant Rabbit Monoclonal Antibody [PSH01-63] - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
