PIP5K1C Recombinant Rabbit Monoclonal Antibody [PSH01-43] - BSA and Azide free
Catalog# HA750720
PIP5K1C Recombinant Rabbit Monoclonal Antibody [PSH01-43] - BSA and Azide free
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WB
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Human
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unconjugated
Overview
Product Name
PIP5K1C Recombinant Rabbit Monoclonal Antibody [PSH01-43] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human PIP5K1C aa 31-470 / 668.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 73 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, 293T cell lysate, A431 cell lysate, SiHa cell lysate, A549 cell lysate, LoVo cell lysate.
Conjugation
unconjugated
Clone Number
PSH01-43
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma is an enzyme that in humans is encoded by the PIP5K1C gene. This gene encodes a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus.
Background References
1. Qu M et al. Pip5k1c Loss in Chondrocytes Causes Spontaneous Osteoarthritic Lesions in Aged Mice. Aging Dis. 2023 Apr
2. Roy A et al. PIP5K1C phosphoinositide kinase deficiency distinguishes PIKFYVE-dependent cancer cells from non-malignant cells. Autophagy. 2023 Mar
Subcellular Location
Cell membrane, Endomembrane system, Cytoplasm, Cell junction, focal adhesion, adherens junction, Cell projection, ruffle membrane, phagocytic cup, uropodium; Cytoplasm, Nucleus.
UNIPROT
Synonyms
Diphosphoinositide kinase antibody
LCCS3 antibody
Phosphatidylinositol-4-phosphate 5-kinase type I gamma antibody
Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma antibody
PI51C antibody
PI51C_HUMAN antibody
PIP5K GAMMA antibody
PIP5K1-gamma antibody
Pip5k1c antibody
PIP5KIgamma antibody
ExpandImages
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This data was developed using HA721681, the same antibody clone in a different buffer formulation.
Western blot analysis of PIP5K1C on different lysates with Rabbit anti-PIP5K1C antibody (HA721681) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: 293T cell lysate
Lane 4: A431 cell lysate
Lane 5: SiHa cell lysate
Lane 6: A549 cell lysate
Lane 7: LoVo cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 87/90 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721681) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
This data was developed using HA721681, the same antibody clone in a different buffer formulation.
Western blot analysis of PIP5K1C on different lysates with Rabbit anti-PIP5K1C antibody (HA721681) at 1/1,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si PIP5K1C cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 87 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721681) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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