PP2A alpha + beta Mouse Monoclonal Antibody [A0-G7]
Catalog# M1603-4
PP2A alpha + beta Mouse Monoclonal Antibody [A0-G7]
-
WB
-
IF-Cell
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
PP2A alpha + beta Mouse Monoclonal Antibody [A0-G7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human PP2A alpha + beta aa 260-309 / 309.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 36 kDa
Positive Control
A431 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, F9 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, Hela, MCF-7, SHG-44.
Conjugation
unconjugated
Clone Number
A0-G7
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:2,000-1:5,000
-
IF-Cell
-
1:100-1:500
-
FC
-
1:50-1:200
Target
Function
The catalytic subunit of protein phosphatase 2A (PP2A) is inactivated by in vitro phosphorylation of Tyr-307 by receptor and nonreceptor protein tyrosine kinases. The catalytic subunit of PP2A is phosphorylated by tyrosine-specific protein kinases and associates with a variety of regulatory subunits. Phosphorylation is enhanced in the presence of the phosphatase inhibitor okadaic acid, consistent with an autodephosphorylation reaction. Phosphorylation is catalyzed by p60v-src, p56lck, epidermal growth factor receptors, and insulin receptors. Transient deactivation of PP2A might enhance transmission of cellular signals through kinase cascades within cells. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases.
Background References
1. Lei X et al. PP2A and tumor radiotherapy. Hereditas. 2020 Aug
2. Ronk H et al. Targeting PP2A for cancer therapeutic modulation. Cancer Biol Med. 2022 Nov
Sequence Similarity
Belongs to the PPP phosphatase family. PP-1 subfamily.
Post-translational Modification
Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.; Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.; May be monoubiquitinated by NOSIP.
Subcellular Location
Cytoplasm, Nucleus, Chromosome, centromere, cytoskeleton, spindle pole.
UNIPROT
Synonyms
PP2A A antibody
PP2A alpha antibody
PP2A B antibody
PP2A beta antibody
PP2A-alpha antibody
PP2A-beta antibody
PP2AA_HUMAN antibody
PP2Aalpha antibody
PP2AB_HUMAN antibody
PP2Abeta antibody
ExpandImages
-
Western blot analysis of PP2A alpha + beta on different lysates with Mouse anti-PP2A alpha + beta antibody (M1603-4) at 1/2,000 dilution.
Lane 1: A431 cell lysate (10 µg/Lane)
Lane 2: 293T cell lysate (10 µg/Lane)
Lane 3: NIH/3T3 cell lysate (10 µg/Lane)
Lane 4: F9 cell lysate (10 µg/Lane)
Lane 5: PC-12 cell lysate (10 µg/Lane)
Lane 6: Mouse brain tissue lysate (20 µg/Lane)
Lane 7: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1603-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining PP2A(alpha+beta) (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining PP2A(alpha+beta) (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining PP2A(alpha+beta) (green) in SHG-44 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
PP2A alpha + beta Recombinant Rabbit Monoclonal Antibody [SN70-08]
Application: WB,IF-Cell,IHC-P
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
Products with the same target and pathway