PSMA7 / HSPC Recombinant Rabbit Monoclonal Antibody [PSH14-09]
Catalog# HA723599
PSMA7 / HSPC Recombinant Rabbit Monoclonal Antibody [PSH14-09]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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Green monkey
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HA751504
不含抗保成分
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unconjugated
Overview
Product Name
PSMA7 / HSPC Recombinant Rabbit Monoclonal Antibody [PSH14-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human PSMA7 aa 51-248.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 28 kDa
Positive Control
A549 cell lysate, HepG2 cell lysate, HeLa cell lysate, COS-1 cell lysate, 4T1 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, A549, human liver cancer tissue, human colon tissue, mouse colon tissue.
Conjugation
unconjugated
Clone Number
PSH14-09
Reactivity Data
| WB | IF-Cell | IHC-P | FC | |
|---|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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| Green Monkey |
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:50,000
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IF-Cell
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1:100
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IHC-P
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1:200-1:1,000
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FC
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1:1,000
Target
Function
Proteasome subunit alpha type-7 also known as 20S proteasome subunit alpha-4 is a protein that in humans is encoded by the PSMA7 gene. This protein is one of the 17 essential subunits (alpha subunits 1–7, constitutive beta subunits 1–7, and inducible subunits including beta1i, beta2i, beta5i) that contributes to the complete assembly of 20S proteasome complex. The eukaryotic proteasome recognized degradable proteins, including damaged proteins for protein quality control purpose or key regulatory protein components for dynamic biological processes. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. As a component of alpha ring, proteasome subunit alpha type-7 contributes to the formation of heptameric alpha rings and substrate entrance gate. Importantly, this subunit plays a critical role in the assembly of 19S base and 20S. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension.
Background References
1. Jiao QH et al. PSMA7 promotes the malignant proliferation of esophageal cancer. Heliyon. 2023 Dec
2. Sheng G et al. Pan-caner analysis identifies PSMA7 as a targets for amplification at 20q13.33 in tumorigenesis. Sci Rep. 2024 Feb
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
C6 antibody
HSPC antibody
MGC3755 antibody
OTTHUMP00000031449 antibody
OTTHUMP00000031450 antibody
OTTHUMP00000031453 antibody
Proteasome (prosome macropain) subunit alpha type 7 antibody
Proteasome alpha 7 subunit antibody
Proteasome subunit alpha 4 antibody
Proteasome subunit alpha type 7 antibody
ExpandImages
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Western blot analysis of PSMA7 / HSPC on different lysates with Rabbit anti-PSMA7 / HSPC antibody (HA723599) at 1/50,000 dilution.
Lane 1: A549 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HeLa cell lysate
Lane 4: COS-1 cell lysate
Lane 5: 4T1 cell lysate
Lane 6: Neuro-2a cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723599) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A549 cells labeling PSMA7 / HSPC with Rabbit anti-PSMA7 / HSPC antibody (HA723599) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PSMA7 / HSPC antibody (HA723599) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-PSMA7 / HSPC antibody (HA723599) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723599) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PSMA7 / HSPC antibody (HA723599) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723599) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PSMA7 / HSPC antibody (HA723599) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723599) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of A549 cells labeling PSMA7 / HSPC.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723599, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
PSMA7 / HSPC Recombinant Rabbit Monoclonal Antibody [PSH14-09] - BSA and Azide free
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
