PSMC6 Rabbit Polyclonal Antibody
Catalog# HA500359
PSMC6 Rabbit Polyclonal Antibody
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WB
-
IF-Cell
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IHC-P
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Human
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Mouse
-
Rat
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unconjugated
Overview
Product Name
PSMC6 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human PSMC6 aa 1-200 / 389.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 44 kDa
Positive Control
HepG2 cell lysate, PC-12 cell lysate, rat kidney tissue lysate, 293T cell lysate, SH-SY5Y cell lysate, mouse heart tissue lysate, mouse kidney tissue lysate, SH-SY5Y, rat hippocampus tissue, rat brain tissue, human kidney clear cell carcinoma tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:1,000
-
IF-Cell
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1:100
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IHC-P
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1:1,000
Target
Function
26S protease regulatory subunit S10B, also known as 26S proteasome AAA-ATPase subunit Rpt4, is an enzyme that in humans is encoded by the PSMC6 gene. As the degradation machinery that is responsible for ~70% of intracellular proteolysis, proteasome complex (26S proteasome) plays a critical roles in maintaining the homeostasis of cellular proteome. Accordingly, misfolded proteins and damaged protein need to be continuously removed to recycle amino acids for new synthesis; in parallel, some key regulatory proteins fulfill their biological functions via selective degradation; furthermore, proteins are digested into peptides for MHC class I antigen presentation. To meet such complicated demands in biological process via spatial and temporal proteolysis, protein substrates have to be recognized, recruited, and eventually hydrolyzed in a well controlled fashion. Thus, 19S regulatory particle pertains a series of important capabilities to address these functional challenges. To recognize protein as designated substrate, 19S complex has subunits that are capable to recognize proteins with a special degradative tag, the ubiquitinylation. It also have subunits that can bind with nucleotides (e.g., ATPs) in order to facilitate the association between 19S and 20S particles, as well as to cause confirmation changes of alpha subunit C-terminals that form the substate entrance of 20S complex.
Background References
1. Zhang JY. et. al. The Silence of PSMC6 Inhibits Cell Growth and Metastasis in Lung Adenocarcinoma. Biomed Res Int. 2021 Jun
2. Zhang Y. et. al. PSMC6 promotes osteoblast apoptosis through inhibiting PI3K/AKT signaling pathway activation in ovariectomy-induced osteoporosis mouse model. J Cell Physiol. 2020 Jul
Subcellular Location
Nucleus, Cytoplasm.
Synonyms
26S protease regulatory subunit 10B antibody
26S protease regulatory subunit S10B antibody
26S proteasome AAA-ATPase subunit RPT4 antibody
CADP44 antibody
P44 antibody
Proteasome 26S subunit ATPase 6 antibody
Proteasome subunit p42 antibody
PRS10_HUMAN antibody
PSMC6 antibody
Rpt4 antibody
ExpandImages
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Western blot analysis of PSMC6 on different lysates with Rabbit anti-PSMC6 antibody (HA500359) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: PC-12 cell lysate (10 µg/Lane)
Lane 3: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500359) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of PSMC6 on different lysates with Rabbit anti-PSMC6 antibody (HA500359) at 1/1,000 dilution.
Lane 1: 293T cell lysate (10 µg/Lane)
Lane 2: SH-SY5Y cell lysate (10 µg/Lane)
Lane 3: Mouse heart tissue lysate (20 µg/Lane)
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500359) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of SH-SY5Y cells labeling PSMC6 with Rabbit anti-PSMC6 antibody (HA500359) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMC6 antibody (HA500359) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-PSMC6 antibody (HA500359) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500359) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSMC6 antibody (HA500359) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500359) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney clear cell carcinoma tissue with Rabbit anti-PSMC6 antibody (HA500359) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500359) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
PSMC6 Recombinant Rabbit Monoclonal Antibody [PSH0-12]
Application: WB,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
PSMC6 Recombinant Rabbit Monoclonal Antibody [PSH0-12]
Application: WB,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
