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Peroxiredoxin 6 Recombinant Mouse Monoclonal Antibody [7G1-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610174

Peroxiredoxin 6 Recombinant Mouse Monoclonal Antibody [7G1-R] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Peroxiredoxin 6 Recombinant Mouse Monoclonal Antibody [7G1-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant full length protein of Human PRDX6.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 25 kDa

Positive Control

HeLa cell lysate, K-562 cell lysate, mouse lung tissue lysate, mouse liver tissue lysate, rat lung tissue lysate, rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

7G1-R

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

Peroxiredoxin-6 is a protein that in humans is encoded by the PRDX6 gene. It is a member of the peroxiredoxin family of antioxidant enzymes. Peroxiredoxin 6 is widely distributed in several organs, especially the lungs. The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.

Background References

1. Kang S W et al. Characterization of a mammalian peroxiredoxin that contains one conserved cysteine. J Biol Chem 273:6303-6311 (1998).

2. Chen J-W et al. 1-Cys peroxiredoxin, a bifunctional enzyme with glutathione peroxidase and phospholipase A2 activities. J Biol Chem 275:28421-28427 (2000).

Subcellular Location

Cytoplasm. Lysosome.

UNIPROT

SwissProt: P30041 Human

SwissProt: O08709 Mouse

SwissProt: O35244 Rat

Synonyms

1 Cys antibody

1 Cys peroxiredoxin antibody

1 Cys PRX antibody

1 cysPrx antibody

1-Cys peroxiredoxin antibody

1-Cys PRX antibody

24 kDa protein antibody

9430088D19Rik antibody

AA690119 antibody

Acidic calcium independent phospholipase A2 antibody

Expand

Images

  • Western blot analysis of Peroxiredoxin 6 on different lysates with Mouse anti-Peroxiredoxin 6 antibody (<a href="/products/HA610174" style="font-weight: bold;text-decoration: underline;">HA610174</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: K-562 cell lysate (20 µg/Lane)<br />Lane 3: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 5: Rat lung tissue lysate (40 µg/Lane)<br />Lane 6: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 25 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 2 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610174" style="font-weight: bold;text-decoration: underline;">HA610174</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Peroxiredoxin 6 on different lysates with Mouse anti-Peroxiredoxin 6 antibody (HA610174) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: K-562 cell lysate (20 µg/Lane)
    Lane 3: Mouse lung tissue lysate (40 µg/Lane)
    Lane 4: Mouse liver tissue lysate (40 µg/Lane)
    Lane 5: Rat lung tissue lysate (40 µg/Lane)
    Lane 6: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 25 kDa
    Observed band size: 27 kDa

    Exposure time: 2 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610174) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Peroxiredoxin 6 on different lysates with Mouse anti-Peroxiredoxin 6 antibody (<a href="/products/HA610174" style="font-weight: bold;text-decoration: underline;">HA610174</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Peroxiredoxin 6 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 25 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 21 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610174" style="font-weight: bold;text-decoration: underline;">HA610174</a>) at 1/2,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Peroxiredoxin 6 on different lysates with Mouse anti-Peroxiredoxin 6 antibody (HA610174) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Peroxiredoxin 6 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 25 kDa
    Observed band size: 27 kDa

    Exposure time: 21 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610174) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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