Phospho-AKT2 (S474) Recombinant Rabbit Monoclonal Antibody [PSH09-46] - BSA and Azide free
Catalog# HA751293
Phospho-AKT2 (S474) Recombinant Rabbit Monoclonal Antibody [PSH09-46] - BSA and Azide free
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WB
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FC
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Human
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Mouse
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Rat
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HA723108
含抗保成分
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unconjugated
Overview
Product Name
Phospho-AKT2 (S474) Recombinant Rabbit Monoclonal Antibody [PSH09-46] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser474 of human AKT2.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 56 kDa
Positive Control
MCF7 cell lysate, MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate, HEK-293 cell lysate, HEK-293 treated with 50μM LY294002 for 6 hours cell lysate, NIH/3T3 treated with 100ng/mL PDGF for 1 hour cell lysate, C6 cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate, MCF7 cells treated with 50ng/mL Calyculin A for 45 minutes, C6 cells treated with 100ng/mL Calyculin A for 1 hours.
Conjugation
unconjugated
Clone Number
PSH09-46
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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FC
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1:1,000
Target
Function
AKT2, also known as RAC-beta serine/threonine-protein kinase, is an enzyme that in humans is encoded by the AKT2 gene. It influences metabolite storage as part of the insulin signal transduction pathway. This gene is a putative oncogene encoding a protein belonging to the AKT subfamily of serine/threonine kinases that contain SH2-like (Src homology 2-like) domains. The encoded protein is a general protein kinase capable of phosphorylating several known proteins. AKT2 has important roles in controlling glycogenesis, gluconeogenesis, and glucose transport as part of the insulin signal transduction pathway.
Background References
1. Feng SY et al. Increased joint loading induces subchondral bone loss of the temporomandibular joint via the RANTES-CCRs-Akt2 axis. JCI Insight. 2022 Nov
2. Ghosh S et al. The AKT2/SIRT5/TFEB pathway as a potential therapeutic target in atrophic AMD. bioRxiv [Preprint]. 2023 Aug
Subcellular Location
Cytoplasm, Nucleus, Cell membrane, Early endosome.
Synonyms
AKT antibody
Akt2 antibody
AKT2_HUMAN antibody
HIHGHH antibody
Murine thymoma viral (v-akt) homolog 2 antibody
murine thymoma viral (v-akt) homolog-2 antibody
Oncogene AKT2 protein kinase B beta antibody
PKB antibody
PKB beta antibody
PKBB antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-AKT2 (S474) on different lysates with Rabbit anti-Phospho-AKT2 (S474) antibody (HA751293) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: HEK-293 treated with 50μM LY294002 for 6 hours cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: NIH/3T3 treated with 100ng/mL PDGF for 1 hour cell lysate
Lane 7: C6 cell lysate
Lane 8: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: Lane 1-6: 3 minutes; Lane 7-8: 12 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751293) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Flow cytometric analysis of MCF7 cells (left) / MCF7 cells treated with 50ng/mL Calyculin A for 45 minutes (right) labeling Phospho-AKT2 (S474).
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751293, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
☑ Cell treatment (CT)
Flow cytometric analysis of C6 cells (left) / C6 cells treated with 100ng/mL Calyculin A for 1 hours (right) labeling Phospho-AKT2 (S474).
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751293, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Phospho-AKT2 (S474) Recombinant Rabbit Monoclonal Antibody [PSH09-46]
Application: WB,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Phospho-AKT2 (S474) Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Mouse
Conjugate: unconjugated
AKT2 Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
