Phospho-CBL (S669) Recombinant Rabbit Monoclonal Antibody [PSH26-91]

Function

Cbl (named after Casitas B-lineage Lymphoma) is a mammalian gene family. CBL gene, a part of the Cbl family, encodes the protein CBL which is an E3 ubiquitin-protein ligase involved in cell signalling and protein ubiquitination. Mutations to this gene have been implicated in a number of human cancers, particularly acute myeloid leukaemia. Ubiquitination is the process of chemically attaching ubiquitin monomers to a protein, thereby targeting it for degradation. As this is a multi-step process, several different enzymes are involved, the final one being a member of the E3 family of ligases. Cbl functions as an E3 ligase, and therefore is able to catalyse the formation of a covalent bond between ubiquitin and Cbl's protein substrate - typically a receptor tyrosine kinase. The RING-finger domain mediates this transfer, however like other E3 ligases of the RING type no intermediate covalent bond is formed between ubiquitin and the RING-finger domain. The stepwise attachment of ubiquitin to the substrate receptor tyrosine kinase can lead to its removal from the plasma membrane and subsequent trafficking to the lysosome for degradation.

Background References

1. Leardini D et al. Role of CBL Mutations in Cancer and Non-Malignant Phenotype. Cancers (Basel). 2022 Feb

2. Pinilla-Macua I et al. Cbl and Cbl-b independently regulate EGFR through distinct receptor interaction modes. Mol Biol Cell. 2023 Dec

Subcellular Location

Cytoplasm, Cell membrane, Cell projection, cilium, Golgi apparatus.

UNIPROT

SwissProt: P22681 Human

SwissProt: P22682 Mouse

Entrez Gene: 500985 Rat

Synonyms