Phospho-HER2 / ErbB2 (T686) Mouse Monoclonal Antibody [4G3]

Function

Neu (ErbB-2 erythroblastic leukemia viral oncogene homolog 2, HER-2, NGL, TKR1, c-erb B2) oncogene was originally cloned from a rat neuroglioblastoma. Human Neu is referred to as HER-2 since the protein structure resembles human epidermal growth factor receptor (HER). ErbB-2 refers to a high level of similarity to ErbB (avian erythroblastosis oncogene B), later found to code for EGFR (HER). Tyr 1248-phosphorylated Neu localizes with Mucin 4/sialomucin complex at the apical surfaces of ductal and alveolar cells in rodent lactating gland. Phosphorylation of Neu at Tyr 1139 promotes association of GRB2 and GRB7 through an Src homology 2 (SH2) domain-dependent interaction and contributes to the etiology of certain breast, gastric and esophageal cancers and testicular germ cell tumors. Neu phosphorylation on Tyr 1221 and Tyr 1248 promotes association of Shc (SH2 domain-containing transforming protein 1) through an SH2 domain. Neu phosphorylation at Tyr 1196 and Tyr 1248 promotes association of Shc through a PTB (phosphotyrosine binding) domain. SH2 and PTB domains recognize tyrosine phosphorylated proteins in a sequence-specific fashion and transduce extracellular signals via subcellular targeting, directing assembly of complexes and modulating enzymatic activity.

Background References

1. Xia, W., et al. 2004. Phosphorylation/cytoplasmic localization of p21Cip1/ WAF1 is associated with HER2/Neu overexpression and provides a novel combination predictor for poor prognosis in breast cancer patients. Clin. Cancer Res. 10: 3815-3824.

2. Thor, A.D., et al. 2000. Activation (tyrosine phosphorylation) of ErbB-2 (HER-2/Neu): a study of incidence and correlation with outcome in breast cancer. J. Clin. Oncol. 18: 3230-3239.

Subcellular Location

Cytoplasm, Cell membrane, Nucleus

UNIPROT

SwissProt: P04626 Human

Synonyms

Receptor tyrosine-protein kinase erbB-2 ERBB2 antibody

Receptor tyrosine-protein kinase erbB-2 HER2 antibody

Receptor tyrosine-protein kinase erbB-2 MLN19 antibody

Receptor tyrosine-protein kinase erbB-2 NEU antibody