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Phospho-Lamin A + Lamin C (S22) Recombinant Rabbit Monoclonal Antibody [PSH11-48] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751410

Phospho-Lamin A + Lamin C (S22) Recombinant Rabbit Monoclonal Antibody [PSH11-48] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Phospho-Lamin A + Lamin C (S22) Recombinant Rabbit Monoclonal Antibody [PSH11-48] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser22 of Human Lamin A + Lamin C.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 74 kDa

Positive Control

HeLa cell lysate, HeLa treated with 100nM paclitaxel for 20 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate, C6 cell lysate, C6 treated with 4mM hydroxyurea for 20 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH11-48

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Prelamin-A/C, or lamin A/C is a protein that in humans is encoded by the LMNA gene. Lamin A/C belongs to the lamin family of proteins. DNA double-strand damages can be repaired by either homologous recombination (HR) or non-homologous end joining (NHEJ). LMNA promotes genetic stability by maintaining the levels of proteins that have key roles in HR and NHEJ. Mouse cells that are deficient for maturation of prelamin A have increased DNA damage and chromosome aberrations, and show increased sensitivity to DNA damaging agents. In progeria, the inadequacy of DNA repair, due to defective LMNA, may cause features of premature aging (see DNA damage theory of aging).

Background References

1. Kovacs MT et. al. DNA damage induces nuclear envelope rupture through ATR-mediated phosphorylation of lamin A/C. Mol Cell. 2023 Oct

2. Yamada S et al. TEAD1 trapping by the Q353R-Lamin A/C causes dilated cardiomyopathy. Sci Adv. 2023 Apr

Subcellular Location

Nucleus lamina, Nucleus envelope, nucleoplasm, Nucleus matrix; Nucleus speckle.

UNIPROT

SwissProt: P02545 Human

SwissProt: P48678 Mouse

SwissProt: P48679 Rat

Synonyms

70 kDa lamin antibody

Cardiomyopathy dilated 1A (autosomal dominant) antibody

CDCD1 antibody

CDDC antibody

CMD1A antibody

CMT2B1 antibody

EMD2 antibody

FPL antibody

FPLD antibody

FPLD2 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Lamin A + Lamin C (S22) on different lysates with Rabbit anti-Phospho-Lamin A + Lamin C (S22) antibody (<a href="/products/HA751410" style="font-weight: bold;text-decoration: underline;">HA751410</a>) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (<a href="/products/ET7110-12" style="font-weight: bold;text-decoration: underline;">ET7110-12</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with 100nM paclitaxel for 20 hours cell lysate<br />Lane 3: NIH/3T3 cell lysate<br />Lane 4: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: C6 treated with 4mM hydroxyurea for 20 hours cell lysate<br />Lane 7: HeLa treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour<br />Lane 8: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour<br />Lane 9: C6 treated with 4mM hydroxyurea for 20 hours cell lysate, then the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 70/65 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751410" style="font-weight: bold;text-decoration: underline;">HA751410</a>) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (<a href="/products/ET7110-12" style="font-weight: bold;text-decoration: underline;">ET7110-12</a>) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Lamin A + Lamin C (S22) on different lysates with Rabbit anti-Phospho-Lamin A + Lamin C (S22) antibody (HA751410) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with 100nM paclitaxel for 20 hours cell lysate
    Lane 3: NIH/3T3 cell lysate
    Lane 4: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate
    Lane 5: C6 cell lysate
    Lane 6: C6 treated with 4mM hydroxyurea for 20 hours cell lysate
    Lane 7: HeLa treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour
    Lane 8: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour
    Lane 9: C6 treated with 4mM hydroxyurea for 20 hours cell lysate, then the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 70/65 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751410) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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