Phospho-PKR (T451) Recombinant Rabbit Monoclonal Antibody [JE46-70]
Catalog# HA722067
Phospho-PKR (T451) Recombinant Rabbit Monoclonal Antibody [JE46-70]
-
WB
-
Human
-
unconjugated
Overview
Product Name
Phospho-PKR (T451) Recombinant Rabbit Monoclonal Antibody [JE46-70]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding Thr45 of human PKR protein.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 62 kDa
Positive Control
HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate, HeLa cells treated with 10ng/mL IFN-α1 for 18 hours then add 100nM Calyculin A for 15 minutes.
Conjugation
unconjugated
Clone Number
JE46-70
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Protein kinase RNA-activated also known as protein kinase R (PKR), interferon-induced, double-stranded RNA-activated protein kinase, or eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2) is an enzyme that in humans is encoded by the EIF2AK2 gene on chromosome 2. PKR is a serine/tyrosine kinase that is 551 amino acids long. PKR is inducible by various mechanisms of stress and protects against viral infections. It also has a role in several signaling pathways.
Background References
1. Liu CX et al. RNA circles with minimized immunogenicity as potent PKR inhibitors. Mol Cell. 2022 Jan
2. Cesaro T et al. Inhibition of PKR by Viruses. Front Microbiol. 2021 Oct
Subcellular Location
Cytoplasm, Nucleus, perinuclear region.
UNIPROT
Synonyms
Double stranded RNA activated protein kinase
antibody
E2AK2_HUMAN antibody
eIF-2A protein kinase 2 antibody
EIF2AK1 antibody
EIF2AK2 antibody
Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
HGNC:9437 antibody
Interferon induced double stranded RNA activated protein kinase antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-PKR (T451) on different lysates with Rabbit anti-Phospho-PKR (T451) antibody (HA722067) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate
Lane 3: HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722067) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Phospho-PKR (T446) Recombinant Rabbit Monoclonal Antibody [SY230] - BSA and Azide free
Application: WB,IHC-P,IP,IF-Cell
Reactivity: Human
Conjugate: unconjugated
PKR Recombinant Rabbit Monoclonal Antibody [SC06-37]
Application: WB
Reactivity: Human
Conjugate: unconjugated
