Phospho-Paxillin (Y118) Recombinant Rabbit Monoclonal Antibody [JE09-10]
Catalog# HA722526
Phospho-Paxillin (Y118) Recombinant Rabbit Monoclonal Antibody [JE09-10]
-
WB
-
Human
-
unconjugated
Overview
Product Name
Phospho-Paxillin (Y118) Recombinant Rabbit Monoclonal Antibody [JE09-10]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr118 of human Paxillin.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 65 kDa
Positive Control
A431 cell lysate, PANC-1 cell lysate.
Conjugation
unconjugated
Clone Number
JE09-10
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Paxillin is a protein that in humans is encoded by the PXN gene. Paxillin is expressed at focal adhesions of non-striated cells and at costameres of striated muscle cells, and it functions to adhere cells to the extracellular matrix. Mutations in PXN as well as abnormal expression of paxillin protein has been implicated in the progression of various cancers.
Background References
1. Ripamonti M et al. Paxillin: A Hub for Mechano-Transduction from the beta3 Integrin-Talin-Kindlin Axis. Front Cell Dev Biol. 2022 Apr
2. Zhang Y et al. Direct active Fyn-paxillin interaction regulates vascular smooth muscle cell migration. J Smooth Muscle Res. 2023
Subcellular Location
Cytoplasm, cytoskeleton, Cell junction, focal adhesion, Cytoplasm, cell cortex.
UNIPROT
Synonyms
FLJ16691 antibody
FLJ23042 antibody
Paired box protein Pax 1 antibody
PAX 1 antibody
PAX1 antibody
PAXI_HUMAN antibody
Paxillin alpha antibody
Paxillin antibody
PXN antibody
PXN protein antibody
Images
-
Western blot analysis of Phospho-Paxillin (Y118) on different lysates with Rabbit anti-Phospho-Paxillin (Y118) antibody (HA722526) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: PANC-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722526) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Phospho-Paxillin (Y118) on different lysates with Rabbit anti-Phospho-Paxillin (Y118) antibody (HA722526) at 1/1,000 dilution and pan Paxillin antibody (ET1607-22) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: A431 cell lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 10 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722526) at 1/1,000 dilution and pan Paxillin antibody (ET1607-22) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Paxillin Recombinant Rabbit Monoclonal Antibody [SY23-02] - BSA and Azide free
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Paxillin Rabbit Polyclonal Antibody
Application: WB,IP,IF
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
