Phospho-SHIP (Y1020) Recombinant Rabbit Monoclonal Antibody [JJ082-03]
Catalog# ET1701-5
Phospho-SHIP (Y1020) Recombinant Rabbit Monoclonal Antibody [JJ082-03]
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WB
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IF-Cell
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IP
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Human
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unconjugated
Overview
Product Name
Phospho-SHIP (Y1020) Recombinant Rabbit Monoclonal Antibody [JJ082-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Tyr1020 of Human SHIP aa 991-1040 / 1,189.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IP
Molecular Weight
Predicted band size: 133 kDa
Positive Control
A549, Hela, HepG2.
Conjugation
unconjugated
Clone Number
JJ082-03
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IP
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Use at an assay dependent concentration.
Target
Function
The major translational product of the v-Fms oncogene, originally isolated from the McDonough strain of feline sarcoma virus, has been identified as a glycoprotein with intrinsic tyrosine kinase activity. The v-Fms human cellular homolog, c-Fms, has been molecularly cloned and mapped to band q34 on chromosome 5, and identified as the receptor for hematopoietic ligand, CSF-1. Ligand-induced activation of the intrinsic CSF-1R protein tyrosine kinase triggers its interaction with cytoplasmic effector molecules. One such effector molecule, SHIP-1 p145 (SH2-containing-inositol phosphatase), associates with activated Fms. SHIP-1 contains two phosphotyrosine-binding domains (PTB), a unique amino terminal SH2 domain, a proline-rich region, and two highly conserved motifs found among inositol phosphate 5-phosphatases. SHIP-1 displays both phosphatidylinositol 3,4,5-triphosphate and inositol 1,3,4,5-tetrakisphosphate polyphosphate 5-phosphatase activity. Evidence suggests that SHIP-1 may modulate Ras signaling in addition to inositol signaling pathways.
Background References
1. Background References 1. Honda F et al. The kinase Btk negatively regulates the production of reactive oxygen species and stimulation-induced apoptosis in human neutrophils. Nat Immunol 13:369-78 (2012).
2. McNulty S et al. The host phosphoinositide 5-phosphatase SHIP2 regulates dissemination of vaccinia virus. J Virol 85:7402-10 (2011).
Sequence Similarity
Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.
Tissue Specificity
Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).
Post-translational Modification
Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.
Subcellular Location
Cell membrane, Cytoplasm, Cytoskeleton, Membrane.
UNIPROT
Synonyms
Inositol polyphosphate 5 phosphatase of 145kDa antibody
4 antibody
5-trisphosphate 5-phosphatase 1 antibody
hp51CN antibody
Inositol polyphosphate 5 phosphatase 145kDa antibody
Inositol polyphosphate 5 phosphatase antibody
Inositol polyphosphate-5-phosphatase of 145 kDa antibody
INPP5D antibody
MGC104855 antibody
MGC142140 antibody
ExpandImages
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ICC staining of Phospho-SHIP (Y1020) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of Phospho-SHIP (Y1020) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of Phospho-SHIP (Y1020) in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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