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Phospho-Syk (Y526) Recombinant Rabbit Monoclonal Antibody [PSH23-78]

Usd: 385

Specification

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Catalog# HA724399

Phospho-Syk (Y526) Recombinant Rabbit Monoclonal Antibody [PSH23-78]

Application

  • WB

  • IP

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Phospho-Syk (Y526) Recombinant Rabbit Monoclonal Antibody [PSH23-78]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Tyr526 of Human Syk.

Species Reactivity

Human

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 72 kDa

Positive Control

Ramos treated with 12ug/mL human IgM for 10min cell lysate.

Conjugation

unconjugated

Clone Number

PSH23-78

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • 1-2μg/sample

Target

Function

Tyrosine-protein kinase SYK, also known as spleen tyrosine kinase, is an enzyme which in humans is encoded by the SYK gene. SYK, along with ZAP70, is a member of the Syk family of tyrosine kinases. These cytoplasmic non-receptor tyrosine kinases share a characteristic dual SH2 domain separated by a linker domain. However, activation of SYK relies less on phosphorylation by Src family kinases than ZAP70. SYK and ZAP70 share a common evolutionary origin and split from a common ancestor in the jawed vertebrates. While Syk and ZAP70 are primarily expressed in hematopoietic tissues, a variety of tissues express Syk. Within B and T cells, respectively, Syk and ZAP70 transmit signals from the B-cell receptor and T-cell receptor. Syk plays a similar role in transmitting signals from a variety of cell surface receptors including CD74, Fc receptor, and integrins.

Background References

1. Wang S et al. TREM2 drives microglia response to amyloid-β via SYK-dependent and -independent pathways. Cell. 2022 Oct

2. Ennerfelt H et al. SYK coordinates neuroprotective microglial responses in neurodegenerative disease. Cell. 2022 Oct

Subcellular Location

Cell membrane, Cytoplasm, cytosol.

UNIPROT

SwissProt: P43405 Human

Synonyms

EC 2.7.10.2 antibody

kinase Syk antibody

KSYK antibody

KSYK_HUMAN antibody

p72-Syk antibody

p72syk antibody

Spleen tyrosine kinase antibody

Syk antibody

Tyrosine protein kinase SYK antibody

Tyrosine-protein kinase SYK antibody

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Syk (Y526) on different lysates with Rabbit anti-Phospho-Syk (Y526) antibody (<a href="/products/HA724399" style="font-weight: bold;text-decoration: underline;">HA724399</a>) at 1/5,000 dilution.<br /><br />Lane 1: Ramos (Human Burkitt's lymphoma cells) cell lysate<br />Lane 2: Ramos treated with 12ug/mL human IgM for 10min cell lysate<br />Lane 3: Ramos treated with 12ug/mL human IgM for 10min cell lysate, then the membrane treated with λpp for 1 hour<br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 42 seconds; ECL: K1801<br /><br />Total Syk protein was detected with another antibody (abcam, ab40781)<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA724399" style="font-weight: bold;text-decoration: underline;">HA724399</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 72 kDa<br />Observed band size: 72 kDa

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Syk (Y526) on different lysates with Rabbit anti-Phospho-Syk (Y526) antibody (HA724399) at 1/5,000 dilution.

    Lane 1: Ramos (Human Burkitt's lymphoma cells) cell lysate
    Lane 2: Ramos treated with 12ug/mL human IgM for 10min cell lysate
    Lane 3: Ramos treated with 12ug/mL human IgM for 10min cell lysate, then the membrane treated with λpp for 1 hour
    Lysates/proteins at 20 µg/Lane.
    Exposure time: 42 seconds; ECL: K1801

    Total Syk protein was detected with another antibody (abcam, ab40781)

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724399, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 72 kDa
    Observed band size: 72 kDa

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunoprecipitation (IP)<br /><br />Phospho-Syk (Y526) was immunoprecipitated in 0.2 mg Ramos (Human Burkitt's lymphoma cells) treated with 12ug/mL human IgM for 10min cell lysate with <a href="/products/HA724399" style="font-weight: bold;text-decoration: underline;">HA724399</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA724399" style="font-weight: bold;text-decoration: underline;">HA724399</a> at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Ramos (Human Burkitt's lymphoma cells) treated with 12ug/mL human IgM for 10min cell lysate (input)<br />Lane 2: <a href="/products/HA724399" style="font-weight: bold;text-decoration: underline;">HA724399</a> IP in Ramos (Human Burkitt's lymphoma cells) treated with 12ug/mL human IgM for 10min cell lysate <br />Lane 3: Rabbit IgG instead of <a href="/products/HA724399" style="font-weight: bold;text-decoration: underline;">HA724399</a> in Ramos (Human Burkitt's lymphoma cells) treated with 12ug/mL human IgM for 10min cell lysate <br /><br />Exposure time: 30 seconds<br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary dilution: <a href="/products/HA724399" style="font-weight: bold;text-decoration: underline;">HA724399</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), 2 hours at room temperature<br /><br />Predicted band size: 72 kDa<br />Observed band size: 72 kDa

    ☑ Cell treatment (CT)

    Immunoprecipitation (IP)

    Phospho-Syk (Y526) was immunoprecipitated in 0.2 mg Ramos (Human Burkitt's lymphoma cells) treated with 12ug/mL human IgM for 10min cell lysate with HA724399 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724399 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Ramos (Human Burkitt's lymphoma cells) treated with 12ug/mL human IgM for 10min cell lysate (input)
    Lane 2: HA724399 IP in Ramos (Human Burkitt's lymphoma cells) treated with 12ug/mL human IgM for 10min cell lysate
    Lane 3: Rabbit IgG instead of HA724399 in Ramos (Human Burkitt's lymphoma cells) treated with 12ug/mL human IgM for 10min cell lysate

    Exposure time: 30 seconds
    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary dilution: HA724399, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

    Predicted band size: 72 kDa
    Observed band size: 72 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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