Phospholamban Mouse Monoclonal Antibody [1G3]

Function

The Sarco(endo)plasmic-reticulum (SER) regulatory protein, Phospholamban (PLB), is a small, plasma membrane-associated phospho-protein found in the SER of cardiac, smooth and slow-twitch muscle. Believed to assemble into a pentamer, PLB regulates cardiac contractility and Ca2+ affinity for cardiac SER Ca2+ ATPase (SERCA2a). Non-phosphorylated PLB associates with SERCA2a, and inhibits Ca2+ reuptake into the SER. PLB activation occurs when key Serine/Threonine residues in PLB (Ser 10, Ser 16, Thr 17) are phosphorylated by numerous effectors, which include PKC, PKA, PKG, and CaM kinase. Phosphorylation of PLB causes dissociation from SERCA2a and a subsequent increase in the rate of Ca2+ reuptake into the SER, which accelerates ventricular relaxation.

Background References

1. Zhai, J., et al. 2000. Cardiac-specific overexpression of a superinhibitory pentameric phospholamban mutant enhances inhibition of cardiac function in vivo. J. Biol. Chem. 275: 10538-10544.

2. Minamisawa, S., et al. 1999. Chronic phospholamban-sarcoplasmic reticulum calcium ATPase interaction is the critical calcium cycling defect in dilated cardiomyopathy. Cell 99: 313-322.

Subcellular Location

Sarcoplasmic reticulum membrane Mitochondrion membrane, Endoplasmic reticulum membrane

UNIPROT

SwissProt: P26678 Human

Synonyms

Cardiac phospholamban antibody

CMD1P antibody

CMH18 antibody

PLB antibody

Pln antibody

PPLA_HUMAN antibody