Prostate Specific Antigen Recombinant Rabbit Monoclonal Antibody [PSH20-80]

☑ Relative expression (RE)

Western blot analysis of Prostate Specific Antigen on different lysates with Rabbit anti-Prostate Specific Antigen antibody (HA724175) at 1/5,000 dilution.

Lane 1: LNCaP cell lysate
Lane 2: DU 145 cell lysate (negative)

Lysates/proteins at 15 µg/Lane.

Predicted band size: 29 kDa
Observed band size: 34 kDa

Exposure time: 3 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724175) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

☑ Relative expression (RE)

Immunocytochemistry analysis of LNCaP (positive) and DU 145 (negative) labeling Prostate Specific Antigen with Rabbit anti-Prostate Specific Antigen antibody (HA724175) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Prostate Specific Antigen antibody (HA724175) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.