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FAM38A / PIEZO1 Mouse Monoclonal Antibody [2-10]

Usd: 185

Specification

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Catalog# M1005-2

FAM38A / PIEZO1 Mouse Monoclonal Antibody [2-10]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

FAM38A / PIEZO1 Mouse Monoclonal Antibody [2-10]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human PIEZO1 aa 1,780-1,980 / 2,521.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 287 kD

Positive Control

MCF-7, Hela, Siha, A431, mouse brain tissue, rat brain tissue, rat hippocampus tissue.

Conjugation

unconjugated

Clone Number

2-10

RRID

AB_3073014

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:200-1:600

Target

Function

Pore-forming subunit of a mechanosensitive non-specific cation channel. Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation. Plays a key role in blood vessel formation and vascular structure in both development and adult physiology.

Background References

1. Lee W et al. Synergy between Piezo1 and Piezo2 channels confers high-strain mechanosensitivity to articular cartilage. Proc Natl Acad Sci U S A 111:E5114-22 (2014).

2. Jia S et al. FAM3A promotes vascular smooth muscle cell proliferation and migration and exacerbates neointima formation in rat artery after balloon injury. J Mol Cell Cardiol 74:173-82 (2014).

Sequence Similarity

Belongs to the PIEZO (TC 1.A.75) family.

Tissue Specificity

Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes. Expressed in erythrocytes (at protein level).

Subcellular Location

Endoplasmic reticulum membrane. Cell membrane.

UNIPROT

SwissProt: Q92508 Human

SwissProt: E2JF22 Mouse

SwissProt: Q0KL00 Rat

Synonyms

DHS

Fam38a

Family with sequence similarity 38 member A

KIAA0233

Membrane protein induced by beta-amyloid treatment

Mib

PIEZ1_HUMAN

Piezo-type mechanosensitive ion channel component 1

PIEZO1

Protein FAM38A

Expand

Images

  • Western blot analysis of FAM38A / PIEZO1 on recombinant protein with Mouse anti-FAM38A / PIEZO1 antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 50 ng/Lane.<br /><br />Exposure time: 1 minute;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FAM38A / PIEZO1 on recombinant protein with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/500 dilution.

    Lysates/proteins at 50 ng/Lane.

    Exposure time: 1 minute;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of FAM38A / PIEZO1 on MCF-7 cell lysates with Mouse anti-FAM38A / PIEZO1 antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 287 kDa<br />Observed band size: 287 kDa<br /><br />Exposure time: 2 minutes;<br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FAM38A / PIEZO1 on MCF-7 cell lysates with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 287 kDa
    Observed band size: 287 kDa

    Exposure time: 2 minutes;
    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Hela cells labeling FAM38A / PIEZO1 with Mouse anti-FAM38A / PIEZO1 antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A / PIEZO1 antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Hela cells labeling FAM38A / PIEZO1 with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of Siha cells labeling FAM38A / PIEZO1 with Mouse anti-FAM38A / PIEZO1 antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A / PIEZO1 antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Siha cells labeling FAM38A / PIEZO1 with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of A431 cells labeling FAM38A / PIEZO1 with Mouse anti-FAM38A / PIEZO1 antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A / PIEZO1 antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of A431 cells labeling FAM38A / PIEZO1 with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-FAM38A / PIEZO1 antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1005-2) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-FAM38A / PIEZO1 antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/600 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/600 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1005-2) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-FAM38A / PIEZO1 antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/600 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1005-2" style="font-weight: bold;text-decoration: underline;">M1005-2</a>) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/600 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1005-2) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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FAM38A / PIEZO1 Recombinant Mouse Monoclonal Antibody [2-10-R-A]

Application: WB,IHC-P,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated