CAMK2D Rabbit Polyclonal Antibody
Catalog# R1305-2
CAMK2D Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
CAMK2D Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human CAMK2D aa 421-470 / 499.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 56 kDa
Positive Control
K562,Hela,human brain tissue,Human skeletal muscle tissue
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:50
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IHC-P
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1:200
Target
Function
Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca2+ homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca2+ influx into the myocyte, Ca2+ release from the sarcoplasmic reticulum (SR), SR Ca2+ uptake and Na+ and K+ channel transport. CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other.
Background References
1. "Ca2+-calmodulin-dependent protein kinase expression and signalling in skeletal muscle during exercise." Rose A.J., Kiens B., Richter E.A. J. Physiol. (Lond.) 574:889-903(2006)
2. "Structure of the CaMKIIdelta/calmodulin complex reveals the molecular mechanism of CaMKII kinase activation."Rellos P., Pike A.C., Niesen F.H., Salah E., Lee W.H., von Delft F., Knapp S. PLoS Biol. 8:E1000426-E1000426(2010)
Sequence Similarity
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Tissue Specificity
Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.
Post-translational Modification
Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.
Subcellular Location
Cell membrane
UNIPROT
Synonyms
Calcium / calmodulin dependent protein kinase 2 delta antibody
Calcium / calmodulin dependent protein kinase II delta antibody
calcium/calmodulin-dependent protein kinase (CaM kinase) II delta antibody
calcium/calmodulin-dependent protein kinase type II delta chain antibody
Calcium/calmodulin-dependent protein kinase type II subunit delta antibody
CAM kinase 2 delta antibody
CAM kinase II delta antibody
CaM kinase II delta subunit antibody
CaM kinase II subunit delta antibody
CaM-kinase II delta chain antibody
ExpandImages
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Western blot analysis on K562 using anti-CAMK2D polyclonal antibody
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Immunofluorescent staining of Hela cells using anti- CAMK2D rabbit polyclonal antibody.
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Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti- CAMK2D rabbit polyclonal antibody.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- CAMK2D rabbit polyclonal antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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