Avi tag Rabbit Polyclonal Antibody
Catalog# R1412-1
Avi tag Rabbit Polyclonal Antibody
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WB
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ELISA
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Species independent
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unconjugated
Overview
Product Name
Avi tag Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
This antibody is produced by immunizing rabbits with a synthetic peptide GLNDIFEAQKIEWHE (KLH-coupled).
Species Reactivity
Species independent
Validated Applications
WB, ELISA
Positive Control
Avi- tagged fusion protein.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:10,000
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ELISA
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1:10,000
Target
Function
Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain.
Background References
1. Lam MT.,et al. Rev-Erbs repress macrophage gene expression by inhibiting enhancer-directed transcription.Nature, 2013Jun.
2. Janes PW.,et al. Eph Receptor Function Is Modulated By Heterooligomerization Of A And B Type Eph Receptors. J Cell Biol, 2011, Dec.
3. Sung K.,et al. A novel method for producing mono-biotinylated, biologically active neurotrophic factors: An essential reagent for single molecule study of axonal transport. J Neurosci Methods, 2011, 200(2):121-8.
Images
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Western blot analysis on Avi tagged fusion protein.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"