Rubisco activase Rabbit Polyclonal Antibody
Catalog# R1608-2
Rubisco activase Rabbit Polyclonal Antibody
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WB
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IHC-P
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Arabidopsis thaliana
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Nicotiana tabacum
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Oryza sativa
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Zea mays
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Spinacia oleracea
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Morus alba
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unconjugated
Overview
Product Name
Rubisco activase Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within C-terminal Arabidopsis thaliana Rubisco activase.
Species Reactivity
Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Zea mays, Spinacia oleracea, Morus alba
Validated Applications
WB, IHC-P
Molecular Weight
46/42 kDa
Positive Control
A. thaliana.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:10,000-1:50,000
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IHC-P
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1:50-1:200
Target
Function
Ribulose bisphosphate carboxylase/oxygenase activase is an enzyme localized to chloroplasts which activates Rubisco by promoting ATP-dependent conformational changes. Alternative name: RuBisCo activase RCA. There are two forms of activase (alpha and beta) in some species (for example Arabidopsis, camelina, spinach, rice) and only one form in other species (tobacco, maize,?Chlamydomonas). Alpha is about 46-47 kDa, beta is about 42 kDa. Species that have only one form have the beta form.
Background References
1. Jurczyk et al. Evidence for alternative splicing mechanisms in meadow fescue (Festuca pratensis) and perennial ryegrass (Lolium perenne) Rubisco activase gene. J Plant Physiol 176:61-64 (2015).
2. Yin et al. Characterization of Rubisco activase genes in maize: an α-isoform gene functions alongside a β-isoform gene. Plant Physiol 164(4):2096-106 (2014).
Sequence Similarity
Belongs to the RuBisCO activase family.
Post-translational Modification
Phosphorylated at Thr-78 by CK2.
Subcellular Location
Plastid.
Images
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Western blot analysis on A. thaliana tissue lysates using anti-Rubisco activase rabbit polyclonal antibody at 1/10,000 dilution.
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Immunohistochemical analysis of paraffin-embedded A. thaliana using anti-Rubisco activase rabbit polyclonal antibody.
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Immunohistochemical analysis of paraffin-embedded A. thaliana using anti-Rubisco activase rabbit polyclonal antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"