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RAC1 Recombinant Rabbit Monoclonal Antibody [PSH05-09] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750982

RAC1 Recombinant Rabbit Monoclonal Antibody [PSH05-09] - BSA and Azide free

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

RAC1 Recombinant Rabbit Monoclonal Antibody [PSH05-09] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human RAC1 aa 1-192 / 192.

Species Reactivity

Human, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 21 kDa

Positive Control

HEK-293 cell lysate, Huh7 cell lysate, HUVEC cell lysate, HL-60 cell lysate, HeLa cell lysate, PC-12 cell lysate, HL-60.

Conjugation

unconjugated

Clone Number

PSH05-09

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

Target

Function

Rac1, also known as Ras-related C3 botulinum toxin substrate 1, is a protein found in human cells. It is encoded by the RAC1 gene. This gene can produce a variety of alternatively spliced versions of the Rac1 protein, which appear to carry out different functions. Rac1 is a small (~21 kDa) signalling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of GLUT4 translocation to glucose uptake, cell growth, cytoskeletal reorganization, antimicrobial cytotoxicity, and the activation of protein kinases. Rac1 is a pleiotropic regulator of many cellular processes, including the cell cycle, cell-cell adhesion, motility (through the actin network), and of epithelial differentiation (proposed to be necessary for maintaining epidermal stem cells).

Background References

1. Liu J et al. Metabolic enzyme LDHA activates Rac1 GTPase as a noncanonical mechanism to promote cancer. Nat Metab. 2022 Dec

2. Liang J et al. Rac1, A Potential Target for Tumor Therapy. Front Oncol. 2021 May

Subcellular Location

Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus, Synapse.

UNIPROT

SwissProt: P63000 Human

SwissProt: Q6RUV5 Rat

Synonyms

Cell migration inducing gene 5 protein antibody

Cell migration-inducing gene 5 protein antibody

MGC111543 antibody

MIG5 antibody

Migration inducing gene 5 antibody

Migration inducing protein 5 antibody

p21 Rac1 antibody

p21-Rac1 antibody

Rac 1 antibody

RAC1 antibody

Expand

Images

  • Western blot analysis of RAC1 on different lysates with Rabbit anti-RAC1 antibody (<a href="/products/HA750982" style="font-weight: bold;text-decoration: underline;">HA750982</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: Huh7 cell lysate<br />Lane 3: HUVEC cell lysate<br />Lane 4: HL-60 cell lysate<br />Lane 5: HeLa cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750982" style="font-weight: bold;text-decoration: underline;">HA750982</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RAC1 on different lysates with Rabbit anti-RAC1 antibody (HA750982) at 1/2,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: Huh7 cell lysate
    Lane 3: HUVEC cell lysate
    Lane 4: HL-60 cell lysate
    Lane 5: HeLa cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750982) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of RAC1 on different lysates with Rabbit anti-RAC1 antibody (<a href="/products/HA750982" style="font-weight: bold;text-decoration: underline;">HA750982</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750982" style="font-weight: bold;text-decoration: underline;">HA750982</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RAC1 on different lysates with Rabbit anti-RAC1 antibody (HA750982) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750982) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HL-60 cells labeling RAC1 with Rabbit anti-RAC1 antibody (<a href="/products/HA750982" style="font-weight: bold;text-decoration: underline;">HA750982</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RAC1 antibody (<a href="/products/HA750982" style="font-weight: bold;text-decoration: underline;">HA750982</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HL-60 cells labeling RAC1 with Rabbit anti-RAC1 antibody (HA750982) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RAC1 antibody (HA750982) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of RAC1 on different lysates with Rabbit anti-RAC1 antibody (<a href="/products/HA750982" style="font-weight: bold;text-decoration: underline;">HA750982</a>) at 1/1,000 dilution.<br /><br />Lane 1: Huh7-parental cell lysate<br />Lane 2: Huh7-RAC1 KD cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750982" style="font-weight: bold;text-decoration: underline;">HA750982</a>) at 1/1,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of RAC1 on different lysates with Rabbit anti-RAC1 antibody (HA750982) at 1/1,000 dilution.

    Lane 1: Huh7-parental cell lysate
    Lane 2: Huh7-RAC1 KD cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750982) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

RAC1 Recombinant Rabbit Monoclonal Antibody [PSH05-09]

Application: WB,IF-Cell

Reactivity: Human,Rat

Conjugate: unconjugated