RALA Mouse Monoclonal Antibody
Catalog# EM1707-77
RALA Mouse Monoclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Monkey
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unconjugated
Overview
Product Name
RALA Mouse Monoclonal Antibody
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein
Species Reactivity
Human, Mouse, Monkey
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
24 kDa
Positive Control
Human RALA recombinant protein, RALA-hIgGFc transfected HEK293 cell lysate, HepG2, MCF-7, A549, K562, Raji, MOLT4, Hela, COS7, NIH/3T3, human bladder cancer tissue, human ovarian cancer tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:100-1:200
Target
Function
Ral A and Ral B constitute a distinct subfamily of Ras-related GTPases (i.e., GDP/GTP binding proteins). Ral proteins are activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. Unlike Ras proteins, Ral A and Ral B fail to induce transformed foci when activated variants are expressed in various recipient cells. A potential downstream target of Ral, designated Ral BP-1, has been shown to contain a Rho-GTPase-activating domain. This Rho-GTPase-activating domain interacts preferentially with the Rho family member Cdc42. A Ras/Ral signaling pathway has been reported to mediate phospholipase D (PLD) activation by v-Src, thus indicating PLD as another downstream target of Ral A.
Background References
1. Lianoglou S et al. Ubiquitously transcribed genes use alternative polyadenylation to achieve tissue-specific expression. Genes Dev 27:2380-96 (2013).
2. Zhu X et al. Inhibition of small GTPase RalA regulates growth and arsenic-induced apoptosis in chronic myeloid leukemia (CML) cells.Cell Signal 24(6):1134-40 (2012).
Subcellular Location
Cell surface. Cell membrane. Cleavage furrow.
UNIPROT
Synonyms
MGC48949 antibody
Ral a antibody
Ral A protein antibody
RAL antibody
RALA antibody
RALA_HUMAN antibody
Ras family small GTP binding protein RALA antibody
RAS like protein A antibody
Ras related protein RalA antibody
Ras-related protein Ral-A antibody
ExpandImages
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Western blot analysis of RALA on human RALA recombinant protein using anti-RALA antibody at 1/1,000 dilution.
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Western blot analysis of RALA on HEK293 (1) and RALA-hIgGFc transfected HEK293 (2) cell lysate using anti-RALA antibody at 1/1,000 dilution.
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Western blot analysis of RALA on different cell lysate using anti-RALA antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: MCF-7
Lane 3: A549
Lane 4: K562
Lane 5: Raji
Lane 6: MOLT4
Lane 7: Hela
Lane 8: COS7
Lane 9: NIH/3T3 -
ICC staining RALA (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-RALA antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RALA antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
RALA Recombinant Rabbit Monoclonal Antibody [JE54-12]
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway
RALA Recombinant Rabbit Monoclonal Antibody [JE54-12]
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
