RICTOR Mouse Monoclonal Antibody [E9-B3]
Catalog# EM1709-50
RICTOR Mouse Monoclonal Antibody [E9-B3]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Monkey
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unconjugated
Overview
Product Name
RICTOR Mouse Monoclonal Antibody [E9-B3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human RICTOR aa 1502-1708 / 1708.
Species Reactivity
Human, Mouse, Monkey
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
192 kDa
Positive Control
Hela, PANC-1, MOLT4, HepG2, HEK293, NIH-3T3, human thyroid gland tissue, human breast carcinoma tissue.
Conjugation
unconjugated
Clone Number
E9-B3
Product Features
Form
Liquid
Concentration
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IF-cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:100-1:200
Target
Function
Rapamycin-insensitive companion of FRAP, also designated Rictor, shares homology with pianissimo from D. discoidieum, STE20p from S . pombe, and AVO3p from S. cerevisiae. Rictor forms a complex with FRAP, which is important in cell growth regulation as it integrates growth factor and nutrient derived signals. The Rictor-FRAP complex plays a role in PKC alpha phosphorylation, directly phosphorylates Akt / PKB on Ser473 in vitro and facilitates Thr308 phosphorylation by PDK1. It also may function as a drug target in tumors that have lost expression of PTEN, a tumor suppressor that opposes activation of Akt / PKB.
Background References
暂无
Subcellular Location
Cytosol.
Synonyms
AVO3 antibody
AVO3 homolog antibody
DKFZp686B11164 antibody
hAVO3 antibody
KIAA1999 antibody
Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody
mAVO3 antibody
MGC39830 antibody
PIA antibody
Pianissimo antibody
ExpandImages
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Western blot analysis of RICTOR on different cell lysates using anti-RICTOR antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: PANC-1
Lane 3: MOLT4
Lane 4: HepG2
Lane 5: HEK293 -
ICC staining RICTOR (green) and actin filaments (red) in NIH/3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-RICTOR antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RICTOR antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Streptococcus pneumoniae promotes migration and invasion of A549 cells in vitro by activating mTORC2/AKT through up-regulation of DDIT4 expression
Journal: Frontiers In Microbiology
DOI:
IF: 6.064
Application: WB
Reactivity: Human
Publish date: 2022 Dec
Alternative Products
RICTOR Recombinant Rabbit Monoclonal Antibody [1A5]
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human,Mouse
Conjugate: unconjugated
