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ROBO1 Mouse Monoclonal Antibody [10E2]

Usd: 350

Specification

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Catalog# EM1901-72

ROBO1 Mouse Monoclonal Antibody [10E2]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

ROBO1 Mouse Monoclonal Antibody [10E2]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human ROBO1 aa 41-286 / 1,651.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 181 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cell) cell lysate,HepG2 (Human chronic myelogenous leukemia cell) cell lysate, human breast tissue, human placenta tissue, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

10E2

RRID

AB_3068917

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:1,000

Target

Function

Specific cells in the midline separate the left and right halves of the central nervous system and play many roles in guiding the growth cone behavior. In vertebrate spinal cord, insect abdominal nerves and nematodes, midline cells produce induced cues, such as nectins and slits, respectively, as attractants and repellents. respectively. These cells can serve as gatekeepers to prevent axons from passing through the midline and to guide the growth cone in response to the switching of lead cues beyond the crossing. One such gatherer, Robo, is an axon guidance receptor that defines a new subfamily of proteins from fruit flies to mammalian conserved Ig superfamily proteins. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous manner Non-intersecting axons express high levels of Robo and cross axons to express low levels of Robo and then reach midline and high levels of crossover. Robo1 and Robo2 are two human homologs of Drosophila megalopolis. Robo1 is also homologous to the C. elegans gene sax3, while Robo2 is homologous to zebrafish genes.

Background References

1. Bianchi G et al. ROBO1 Promotes Homing, Dissemination, and Survival of Multiple Myeloma within the Bone Marrow Microenvironment. Blood Cancer Discov. 2021 Jul

2. Xie J et al. Slit2/Robo1 Mitigates DSS-induced Ulcerative Colitis by Activating Autophagy in Intestinal Stem Cell. Int J Biol Sci. 2020 Apr

Sequence Similarity

Belongs to the immunoglobulin superfamily. ROBO family.

Tissue Specificity

Widely expressed, with exception of kidney.

Post-translational Modification

Ubiquitinated. May be deubiquitinated by USP33.

Subcellular Location

Cell membrane, Cell projection, axon, Endoplasmic reticulum-Golgi intermediate compartment membrane, cytoplasm.

UNIPROT

SwissProt: Q9Y6N7 Human

SwissProt: O89026 Mouse

SwissProt: O55005 Rat

Synonyms

Deleted in U twenty twenty antibody

DUTT 1 antibody

DUTT1 antibody

FLJ21882 antibody

H Robo 1 antibody

H-Robo-1 antibody

hRobo 1 antibody

Robo 1 antibody

Robo1 antibody

ROBO1_HUMAN antibody

Expand

Images

  • Western blot analysis of ROBO1 on different lysates with Rabbit anti-ROBO1 antibody (<a href="/products/EM1901-72" style="font-weight: bold;text-decoration: underline;">EM1901-72</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: HepG2 (Human chronic myelogenous leukemia cell) cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/EM1901-72" style="font-weight: bold;text-decoration: underline;">EM1901-72</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 181 kDa<br />Observed band size: 181 kDa

    Western blot analysis of ROBO1 on different lysates with Rabbit anti-ROBO1 antibody (EM1901-72) at 1/1,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: HepG2 (Human chronic myelogenous leukemia cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: EM1901-72, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 181 kDa
    Observed band size: 181 kDa

  • Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-ROBO1 antibody (<a href="/products/EM1901-72" style="font-weight: bold;text-decoration: underline;">EM1901-72</a>) at 1/600 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-72" style="font-weight: bold;text-decoration: underline;">EM1901-72</a>) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-ROBO1 antibody (EM1901-72) at 1/600 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-72) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-ROBO1 antibody (<a href="/products/EM1901-72" style="font-weight: bold;text-decoration: underline;">EM1901-72</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-72" style="font-weight: bold;text-decoration: underline;">EM1901-72</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-ROBO1 antibody (EM1901-72) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-72) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ROBO1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-72" style="font-weight: bold;text-decoration: underline;">EM1901-72</a>, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ROBO1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-72, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-ROBO1 antibody (<a href="/products/EM1901-72" style="font-weight: bold;text-decoration: underline;">EM1901-72</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-72" style="font-weight: bold;text-decoration: underline;">EM1901-72</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-ROBO1 antibody (EM1901-72) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-72) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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