RON Rabbit Polyclonal Antibody
Catalog# R1512-2
RON Rabbit Polyclonal Antibody
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
RON Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within C-terminal human RON.
Species Reactivity
Human
Validated Applications
IF-Cell, IHC-P
Molecular Weight
Predicted band size: 152 kDa
Positive Control
A549, Ags, SW480, human breast tissue, human breast cancer tissue, human stomach cancer tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.
Background References
1. Wang Q et al. RON confers lapatinib resistance in HER2-positive breast cancer cells. Cancer Lett 340:43-50 (2013).
2. Privette Vinnedge, L.M. et al. The DEK oncogene promotes cellular proliferation through paracrine Wnt signaling in Ron receptor-positive breast cancers. Oncogene. 34: 2325-36 (2015).
Subcellular Location
Membrane.
UNIPROT
Synonyms
c met related tyrosine kinase antibody
CD136 antibody
CD136 antigen antibodyCDw136 antibody
Macrophage stimulating 1 receptor (c met related tyrosine kinase) antibody
Macrophage stimulating 1 receptor antibody
Macrophage stimulating protein receptor alpha chain antibody
MACROPHAGE STIMULATING PROTEIN RECEPTOR antibody
Macrophage stimulating protein receptor beta chain antibody
Macrophage-Stimulating 1 Receptor (MST1R) antibody
Macrophage-stimulating protein receptor beta chain antibody
ExpandImages
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ICC staining RON in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining RON in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining RON in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-RON antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-RON antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-RON antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
RON Recombinant Rabbit Monoclonal Antibody [SD2006]
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP
Reactivity: Human
Conjugate: unconjugated
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