Rad51B Rabbit Polyclonal Antibody
Catalog# HA500010
Rad51B Rabbit Polyclonal Antibody
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WB
-
FC
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Human
-
Rat
-
unconjugated
Overview
Product Name
Rad51B Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within C-terminal human Rad51B.
Species Reactivity
Human, Rat
Validated Applications
WB, FC
Molecular Weight
42 kDa
Positive Control
SKBR3 cell lysate, PC-12 cell lysate, SiHa.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
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FC
-
1:500-1:1,000
Target
Function
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway.
Background References
1. Masson J.Y.et al. Identification and purification of two distinct complexes containing the five RAD51 paralogs. Genes Dev. 15:3296-3307(2001) .
Subcellular Location
Nucleus.
UNIPROT
Synonyms
DNA repair protein RAD51 homolog 2 antibody
hREC2 antibody
MGC34245 antibody
OTTHUMP00000212251 antibody
OTTHUMP00000212253 antibody
OTTHUMP00000212254 antibody
OTTHUMP00000212255 antibody
R51H2 antibody
RA51B_HUMAN antibody
RAD51 homolog B (S. cerevisiae) antibody
ExpandImages
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Western blot analysis of Rad51B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500010, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SKBR3 cell lysate
Lane 2: PC-12 cell lysate -
Flow cytometric analysis of SiHa cells labeling Rad51B.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500010, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"