APC6 Recombinant Rabbit Monoclonal Antibody [SD085-8]
Catalog# ET1612-86
APC6 Recombinant Rabbit Monoclonal Antibody [SD085-8]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
APC6 Recombinant Rabbit Monoclonal Antibody [SD085-8]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human APC6 aa 181-247 / 620.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 72 kDa
Positive Control
Mouse brain tissue lysate, rat brain tissue lysate, Hela cells lysates.
Conjugation
unconjugated
Clone Number
SD085-8
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
Target
Function
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.
Background References
1. Chang L., et al. 2014. Molecular architecture and mechanism of the anaphase-promoting complex. Nature 513:388-393.
2. Zhou H., et al. 2013. Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. 12:260-271.
Sequence Similarity
Belongs to the APC6/CDC16 family.
Post-translational Modification
Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
Subcellular Location
Cytoplasm.
Synonyms
ANAPC6 antibody
Anaphase promoting complex subunit 6 antibody
Anaphase-promoting complex subunit 6 antibody
Apc 6 antibody
APC6 antibody
CDC 16 antibody
CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody
Cdc16 antibody
CDC16 homolog antibody
CDC16 protein antibody
ExpandImages
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Western blot analysis of APC6 on different lysates with Rabbit anti-APC6 antibody (ET1612-86) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 66 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-86) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of APC6 on Hela cells lysates using anti-APC6 antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"