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APLP2 Recombinant Rabbit Monoclonal Antibody [JE54-62]

Usd: 385

Specification

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Catalog# ET7110-78

APLP2 Recombinant Rabbit Monoclonal Antibody [JE54-62]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

APLP2 Recombinant Rabbit Monoclonal Antibody [JE54-62]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human APLP2 aa 201-300 / 763.

Species Reactivity

Human, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 87 kDa

Positive Control

Rat brian tissue lysate, human placenta tissue lysate, Hela cell lysate, rat testis tissue, human kidney tissue.

Conjugation

unconjugated

Clone Number

JE54-62

RRID

AB_3071039

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

This gene encodes amyloid precursor- like protein 2 (APLP2), which is a member of the APP (amyloid precursor protein) family including APP, APLP1 and APLP2. This protein is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. This protein interacts with major histocompatibility complex (MHC) class I molecules. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. This protein has been implicated in the pathogenesis of Alzheimer's disease. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Background References

1. Gao L. et. al. Role of APLP2 in the prognosis and clinicopathology of renal cell carcinoma. Oncol Lett. 2019 Jan.

2. Wang X. et. al. APLP2 Modulates JNK-Dependent Cell Migration in Drosophila. Biomed Res Int. 2018 Jul.

Sequence Similarity

Belongs to the APP family.

Tissue Specificity

Expressed in placenta, brain, heart, lung, liver, kidney and endothelial tissues.

Post-translational Modification

The BPTI/Kunitz inhibitor domain is O-glycosylated.

Subcellular Location

Nucleus, cell membrane.

UNIPROT

SwissProt: Q06481 Human

SwissProt: P15943 Rat

Synonyms

amyloid beta (A4) precursor like protein 2 antibody

Amyloid like protein 2 antibody

Amyloid precursor protein homolog HSD 2 antibody

amyloid precursor protein homolog HSD2 antibody

Amyloid protein homolog antibody

Amyloid-like protein 2 antibody

APLP-2 antibody

Aplp2 antibody

APLP2_HUMAN antibody

APPH antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of APLP2 on different lysates with Rabbit anti-APLP2 antibody (<a href="/products/ET7110-78" style="font-weight: bold;text-decoration: underline;">ET7110-78</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-WT cell lysate<br />Lane 2: A549-KD APLP2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 87 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 2 minutes 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7110-78" style="font-weight: bold;text-decoration: underline;">ET7110-78</a>) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of APLP2 on different lysates with Rabbit anti-APLP2 antibody (ET7110-78) at 1/1,000 dilution.

    Lane 1: A549-WT cell lysate
    Lane 2: A549-KD APLP2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 87 kDa
    Observed band size: 100 kDa

    Exposure time: 2 minutes 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-78) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of APLP2 on different lysates with Rabbit anti-APLP2 antibody (<a href="/products/ET7110-78" style="font-weight: bold;text-decoration: underline;">ET7110-78</a>) at 1/500 dilution.<br /><br />Lane 1: Rat brian tissue lysate(20 µg/Lane)<br />Lane 2: Human placenta tissue lysate(20 µg/Lane)<br />Lane 3: Hela cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 87 kDa<br />Observed band size: 100/120 kDa<br /><br />Exposure time: 2 minutes;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7110-78" style="font-weight: bold;text-decoration: underline;">ET7110-78</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of APLP2 on different lysates with Rabbit anti-APLP2 antibody (ET7110-78) at 1/500 dilution.

    Lane 1: Rat brian tissue lysate(20 µg/Lane)
    Lane 2: Human placenta tissue lysate(20 µg/Lane)
    Lane 3: Hela cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 87 kDa
    Observed band size: 100/120 kDa

    Exposure time: 2 minutes;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-78) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-APLP2 antibody (<a href="/products/ET7110-78" style="font-weight: bold;text-decoration: underline;">ET7110-78</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-78" style="font-weight: bold;text-decoration: underline;">ET7110-78</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-APLP2 antibody (ET7110-78) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-APLP2 antibody (<a href="/products/ET7110-78" style="font-weight: bold;text-decoration: underline;">ET7110-78</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-78" style="font-weight: bold;text-decoration: underline;">ET7110-78</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-APLP2 antibody (ET7110-78) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"