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BPI Recombinant Rabbit Monoclonal Antibody [SN06-63]

Usd: 385

Specification

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Catalog# ET1611-6

BPI Recombinant Rabbit Monoclonal Antibody [SN06-63]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Zebrafish

Predicted reactivity

  • Zebrafish

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

BPI Recombinant Rabbit Monoclonal Antibody [SN06-63]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human BPI aa 131-180 / 487.

Species Reactivity

Human, Mouse, Zebrafish (Predicted: Zebrafish)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 54 kDa

Positive Control

THP-1 cell lysate, Jurkat cell lysate, HL-60 cell lysate, Mouse thymus tissue lysate, THP-1.

Conjugation

unconjugated

Clone Number

SN06-63

RRID

AB_3070039

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

Target

Function

The bactericidal permeability increasing protein (BPI) is an antibacterial and endotoxin-neutralizing molecule that is abundant in the granules of polymorphonuclear leukocytes (neutrophil granules). The 31.5-kb-long human BPI gene maps to chromosome 20q11.23-q12, contains 15 exons, and encodes a 456 amino acid protein. Epithelial cells which line mucosal surfaces are the first line of defense against bacterial invasion and infection. BPI localizes to the cell surface of epithelial cells and blocks endotoxin-mediated signaling, thereby protecting mucosal surfaces against gram-negative bacteria and their endotoxin. BPI, lipopolysaccharide binding protein (LBP), phospholipid transfer protein (PLTP), and cholesteryl ester transfer protein (CETP) constitutes a family of functionally related proteins.

Background References

1. Hu, N. et al. 2014. Differential expression of granulopoiesis related genes in neutrophil subsets distinguished by membrane expression of CD177. PloS one. 9: e99671.

2. Ota T., et al. 2004. Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat. Genet. 36:40-45.

Sequence Similarity

Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Tissue Specificity

Restricted to cells of the myeloid series.

Subcellular Location

Secreted, Cytoplasmic granule membrane.

UNIPROT

SwissProt: P17213 Human

SwissProt: Q67E05 Mouse

Synonyms

Bactericidal permeability-increasing protein antibody

bactericidal/permeability-increasing protein antibody

BPI antibody

BPI fold containing family D, member 1 antibody

BPIFD1 antibody

CAP 57 antibody

rBPI antibody

recombinant BPI holoprotein, rBPI antibody

Images

  • Western blot analysis of BPI on different lysates with Rabbit anti-BPI antibody (<a href="/products/ET1611-6" style="font-weight: bold;text-decoration: underline;">ET1611-6</a>) at 1/2,000 dilution.<br /><br />Lane 1: THP-1 cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: HL-60 cell lysate (20 µg/Lane)<br />Lane 4: Mouse thymus tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 54 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-6" style="font-weight: bold;text-decoration: underline;">ET1611-6</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BPI on different lysates with Rabbit anti-BPI antibody (ET1611-6) at 1/2,000 dilution.

    Lane 1: THP-1 cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: HL-60 cell lysate (20 µg/Lane)
    Lane 4: Mouse thymus tissue lysate (40 µg/Lane)

    Predicted band size: 54 kDa
    Observed band size: 60 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-6) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of THP-1 cells labeling BPI with Rabbit anti-BPI antibody (<a href="/products/ET1611-6" style="font-weight: bold;text-decoration: underline;">ET1611-6</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BPI antibody (<a href="/products/ET1611-6" style="font-weight: bold;text-decoration: underline;">ET1611-6</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of THP-1 cells labeling BPI with Rabbit anti-BPI antibody (ET1611-6) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BPI antibody (ET1611-6) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"