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Biglycan Recombinant Rabbit Monoclonal Antibody [JB71-31]

Usd: 385

Specification

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Catalog# ET7107-48

Biglycan Recombinant Rabbit Monoclonal Antibody [JB71-31]

Application

  • WB

  • IHC-P

  • FC

  • IP

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

Biglycan Recombinant Rabbit Monoclonal Antibody [JB71-31]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Biglycan aa 260-368 / 368.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, FC, IP

Molecular Weight

Predicted band size: 42 kDa

Positive Control

Mouse skeletal muscle tissue lysates, mouse heart tissue, human kidney tissue, HepG2.

Conjugation

unconjugated

Clone Number

JB71-31

RRID

AB_3070758

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

Target

Function

Biglycan is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. In humans, biglycan is encoded by the BGN gene[5] which is located on the X chromosome. Biglycan is believed to play a role in the mineralization of bone. Knock-out mice that have had the gene for biglycan suppressed (Bgn -/-) have an osteoporosis-like phenotype with reduced growth rate and lower bone mass than mice that can express biglycan. Biglycan core protein binds to the growth factors BMP-4 and influences its bioactivity. It has also been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan binds to TGF-beta 1.

Background References

1. Meester J A et al. Loss-of-function mutations in the X-linked biglycan gene cause a severe syndromic form of thoracic aortic aneurysms and dissections. Genet Med 19:386-395 (2017).

2. Roughley P J et al. Dermatan sulphate proteoglycans of human articular cartilage. The properties of dermatan sulphate proteoglycans I and II. Biochem J 262:823-827 (1989).

Sequence Similarity

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Tissue Specificity

Found in several connective tissues, especially in articular cartilages.

Post-translational Modification

The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.

Subcellular Location

Secreted, extracellular space, extracellular matrix.

UNIPROT

SwissProt: P21810 Human

SwissProt: P28653 Mouse

Synonyms

BGN antibody

Biglycan antibody

Biglycan proteoglycan antibody

Bone/cartilage proteoglycan I antibody

Dermatan sulphate proteoglycan I antibody

DSPG1 antibody

PG S1 antibody

PG-S1 antibody

PGI antibody

PGS1_HUMAN antibody

Expand

Images

  • Western blot analysis of Biglycan on mouse skeletal muscle tissue lysates with Rabbit anti-Biglycan antibody (<a href="/products/ET7107-48" style="font-weight: bold;text-decoration: underline;">ET7107-48</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 5 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-48" style="font-weight: bold;text-decoration: underline;">ET7107-48</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Biglycan on mouse skeletal muscle tissue lysates with Rabbit anti-Biglycan antibody (ET7107-48) at 1/1,000 dilution.

    Lysates/proteins at 5 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-48) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Biglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-48" style="font-weight: bold;text-decoration: underline;">ET7107-48</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Biglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Biglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-48" style="font-weight: bold;text-decoration: underline;">ET7107-48</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Biglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of Biglycan was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET7107-48" style="font-weight: bold;text-decoration: underline;">ET7107-48</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Biglycan was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-48, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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