Skip to content

CD11a Recombinant Rabbit Monoclonal Antibody [SC56-01]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET1610-1

CD11a Recombinant Rabbit Monoclonal Antibody [SC56-01]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CD11a Recombinant Rabbit Monoclonal Antibody [SC56-01]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CD11a aa 1,135-1,170 / 1,170.

Species Reactivity

Human

Validated Applications

WB, IF-Tissue, IHC-P, IP

Molecular Weight

Predicted band size: 129 kDa

Positive Control

THP-1 cell lysate, human colon tissue, human tonsil tissue, human spleen tissue.

Conjugation

unconjugated

Clone Number

SC56-01

RRID

AB_3069892

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:200-1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Integrins are heterodimers composed of noncovalently associated transmembrane a and b subunits. The 16 a and 8 b subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.

Background References

1. Abramowski P et al. A truncation variant of the cation channel P2RX5 is upregulated during T cell activation. PLoS One 9:e104692 (2014).

2. Takahara M et al. Soluble ICAM-1 secretion and its functional role as an autocrine growth factor in nasal NK/T cell lymphoma cells. Exp Hematol 41:711-8 (2013).

Sequence Similarity

Belongs to the integrin alpha chain family.

Tissue Specificity

Leukocytes.

Post-translational Modification

In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P20701 Human

Synonyms

Lymphocyte function associated antigen, type 1, alpha subunit antibody

Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody

Antigen CD11A antibody

CD 11a antibody

CD11 antigen-like family member A antibody

CD11a antibody

CD11a antigen antibody

Integrin Alpha L antibody

Integrin alpha-L antibody

Integrin gene promoter antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD11a on different lysates with Rabbit anti-CD11a antibody (<a href="/products/ET1610-1" style="font-weight: bold;text-decoration: underline;">ET1610-1</a>) at 1/2,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: K-562 cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 129 kDa<br />Observed band size: 180 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-1" style="font-weight: bold;text-decoration: underline;">ET1610-1</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CD11a on different lysates with Rabbit anti-CD11a antibody (ET1610-1) at 1/2,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: K-562 cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 129 kDa
    Observed band size: 180 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-1) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD11a antibody (<a href="/products/ET1610-1" style="font-weight: bold;text-decoration: underline;">ET1610-1</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-1" style="font-weight: bold;text-decoration: underline;">ET1610-1</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD11a antibody (ET1610-1) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-1" style="font-weight: bold;text-decoration: underline;">ET1610-1</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD11a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-1" style="font-weight: bold;text-decoration: underline;">ET1610-1</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD11a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD11a was immunoprecipitated from 0.2 mg THP-1 cell lysate with <a href="/products/ET1610-1" style="font-weight: bold;text-decoration: underline;">ET1610-1</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/ET1610-1" style="font-weight: bold;text-decoration: underline;">ET1610-1</a> at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (<a href="/products/NBI01H" style="font-weight: bold;text-decoration: underline;">NBI01H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: THP-1 cell lysate (input)<br />Lane 2: <a href="/products/ET1610-1" style="font-weight: bold;text-decoration: underline;">ET1610-1</a> IP in THP-1 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/ET1610-1" style="font-weight: bold;text-decoration: underline;">ET1610-1</a> in THP-1 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 2 seconds; ECL: K1801

    CD11a was immunoprecipitated from 0.2 mg THP-1 cell lysate with ET1610-1 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1610-1 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: THP-1 cell lysate (input)
    Lane 2: ET1610-1 IP in THP-1 cell lysate
    Lane 3: Rabbit IgG instead of ET1610-1 in THP-1 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 2 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"