CTNNA3 Recombinant Rabbit Monoclonal Antibody [JE55-32]
Catalog# ET7111-07
CTNNA3 Recombinant Rabbit Monoclonal Antibody [JE55-32]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CTNNA3 Recombinant Rabbit Monoclonal Antibody [JE55-32]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant fragment within C-terminal Human CTNNA3.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
100 kDa
Positive Control
Rat heart tissue lysate, mouse heart tissue lysate, 293 cell lysate, mouse heart tissue, rat heart tissue.
Conjugation
unconjugated
Clone Number
JE55-32
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:500-1:1,000
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IHC-P
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1:50-1:200
Target
Function
This gene encodes a protein that belongs to the vinculin/alpha-catenin family. The encoded protein plays a role in cell-cell adhesion in muscle cells. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia, familial 13. Alternative splicing results in multiple transcript variants.
Background References
1. He B. et. al. CTNNA3 is a tumor suppressor in hepatocellular carcinomas and is inhibited by miR-425. Oncotarget. 2016 Feb
2. McGeachie MJ. et. al. CTNNA3 and SEMA3D: Promising loci for asthma exacerbation identified through multiple genome-wide association studies. J Allergy Clin Immunol. 2015 Dec
Subcellular Location
Cytoskeleton.
Synonyms
4930429L08Rik antibody
4933408A16 antibody
Alpha catenin like protein antibody
Alpha T catenin antibody
Alpha T-catenin antibody
Cadherin associated protein antibody
Cadherin-associated protein antibody
Catenin alpha 3 antibody
Catenin alpha-3 antibody
Catna3 antibody
ExpandImages
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Western blot analysis of CTNNA3 on different lysates with Rabbit anti-CTNNA3 antibody (ET7111-07) at 1/1,000 dilution.
Lane 1: Rat heart tissue lysate (20 µg/Lane)
Lane 2: Mouse heart tissue lysate (20 µg/Lane)
Lane 3: 293 cell lysate (10 µg/Lane)
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-07) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/5,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-CTNNA3 antibody (ET7111-07) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-07) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-CTNNA3 antibody (ET7111-07) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-07) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"