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Caveolin-2 Recombinant Rabbit Monoclonal Antibody [SY22-05]

Usd: 385

Specification

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Catalog# ET1607-15

Caveolin-2 Recombinant Rabbit Monoclonal Antibody [SY22-05]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Caveolin-2 Recombinant Rabbit Monoclonal Antibody [SY22-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Caveolin-2 aa 1-50 / 162.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Molecular Weight

Predicted band size: 18 kDa

Positive Control

HUVEC, Hela, human cervix uteri tissue, human uterus tissue.

Conjugation

unconjugated

Clone Number

SY22-05

RRID

AB_3069752

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

Target

Function

Caveolae (also known as plasmalemmal vesicles) are 50-100 nM flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3, have been identified as principal components of caveolae. Two forms of caveolin-1, designated a and b, share a distinct but overlapping cellular distribution and differ by an N-terminal 31 amino acid sequence that is absent from the b isoform. Caveolin-1 shares 31% identity with caveolin-2 and 65% identity with caveolin-3 at the amino acid level. Functionally, the three proteins differ in their interactions with heterotrimeric G protein isoforms.

Background References

1. Samhan-Arias AK, et al. 2012. Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons. J Proteomics. . 75: 2934-49.

2. Marques-da-Silva D, et al. 2012. L-type voltage-operated calcium channels, N-methyl-D-aspartate receptors and neuronal nitric-oxide synthase form a calcium/redox nano-transducer within lipid rafts. Biochem Biophys Res Commun. 420: 257-62.

Sequence Similarity

Belongs to the caveolin family.

Tissue Specificity

Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.

Post-translational Modification

Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin (By similarity).

Subcellular Location

Cytoplasm, Nucleus, Membrane.

UNIPROT

SwissProt: P51636 Human

Synonyms

CAV antibody

CAV2 antibody

CAV2_HUMAN antibody

Caveolae protein 20 kD antibody

Caveolin 2 antibody

Caveolin 2 isoform a and b antibody

Caveolin-2 antibody

MGC12294 antibody

OTTHUMP00000025032 antibody

OTTHUMP00000195982 antibody

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Caveolin-2 on different lysates with Rabbit anti-Caveolin-2 antibody (<a href="/products/ET1607-15" style="font-weight: bold;text-decoration: underline;">ET1607-15</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Caveolin-2 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 18 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 120 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1607-15" style="font-weight: bold;text-decoration: underline;">ET1607-15</a>) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Caveolin-2 on different lysates with Rabbit anti-Caveolin-2 antibody (ET1607-15) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Caveolin-2 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 18 kDa
    Observed band size: 20 kDa

    Exposure time: 120 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-15) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of caveolin-2 on HUVEC cell lysates using anti-caveolin-2 antibody at 1/1,000 dilution.

    Western blot analysis of caveolin-2 on HUVEC cell lysates using anti-caveolin-2 antibody at 1/1,000 dilution.

  • ICC staining caveolin-2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining caveolin-2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human cervix uteri tissue using anti-caveolin-2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human cervix uteri tissue using anti-caveolin-2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-caveolin-2 antibody. Counter stained with hematoxylin. <br />

    Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-caveolin-2 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation