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Cellular Apoptosis Susceptibility Recombinant Rabbit Monoclonal Antibody [JU34-33]

Usd: 385

Specification

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Catalog# ET7106-90

Cellular Apoptosis Susceptibility Recombinant Rabbit Monoclonal Antibody [JU34-33]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Cellular Apoptosis Susceptibility Recombinant Rabbit Monoclonal Antibody [JU34-33]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Cellular Apoptosis Susceptibility aa 860-971 / 971.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Molecular Weight

Predicted band size: 110 kDa

Positive Control

SW480 cell lysate, MCF7 cell lysate, SK-Br-3 cell lysate, SiHa cell lysate, PC-3M cell lysate, HeLa cell lysate, Ramos cell lysate, mouse testis tissue lysate, LOVO, PC-3M, SH-SY5Y, human colon carcinoma tissue, mouse testis tissue, LOVO.

Conjugation

unconjugated

Clone Number

JU34-33

RRID

AB_3070708

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:200-1:1,000

  • FC

  • 1:50-1:100

Target

Function

Cellular apoptosis susceptibility protein (CAS), also called Exportin 2, is a 971 amino acid member of the CSE1 family. CAS mediates Importin α re-export from the nucleus to the cytoplasm after import substrates have been released into the nucleoplasm. In the nucleus, CAS binds cooperatively to Importin α and to the GTPase Ran in its GTP-bound (active) form. This complex binds to nucleoporins as it docks to the nuclear pore complex. Once in the cytoplasm, the complex dissociates and Importin α is released and CAS returns to the nuclear compartment and the process begins anew. CAS can be detected highly in proliferating cells. Three isoforms of CAS have been named due to alternative splicing. Isoform 1 is the full length, 971 amino acid protein. Isoform 2 contains an alternative sequence for amino acids 190-195 and is missing amino acids 196-971. Isoform 3 contains an alternative sequence for amino acids 943-945 and is missing amino acids 946-971.

Background References

1. Kutay U et al. Export of importin-alpha from the nucleus is mediated by a specific nuclear transport factor. Cell 90:1061-1071 (1997).

2. Brinkmann U et al. Cloning and characterization of a cellular apoptosis susceptibility gene, the human homologue to the yeast chromosome segregation gene CSE1. Proc Natl Acad Sci USA 92:10427-10431 (1995).

Sequence Similarity

Belongs to the XPO2/CSE1 family.

Tissue Specificity

Detected in brain, placenta, ovary, testis and trachea (at protein level). Widely expressed. Highly expressed in testis and in proliferating cells.

Subcellular Location

Cytoplasm. Nucleus.

UNIPROT

SwissProt: P55060 Human

SwissProt: Q9ERK4 Mouse

Synonyms

CAS antibody

Cellular apoptosis susceptibility protein antibody

Chromosome segregation 1 (yeast homolog) like antibody

Chromosome segregation 1 Like antibody

Chromosome segregation 1 like protein antibody

Chromosome segregation 1-like protein antibody

Chromosome segregation gene CSE1 antibody

CSE 1 antibody

CSE 1 chromosome segregation 1 like antibody

CSE 1 chromosome segregation 1 like protein antibody

Expand

Images

  • Western blot analysis of Cellular Apoptosis Susceptibility on different lysates with Rabbit anti-Cellular Apoptosis Susceptibility antibody (<a href="/products/ET7106-90" style="font-weight: bold;text-decoration: underline;">ET7106-90</a>) at 1/1,000 dilution.<br /><br />Lane 1: SW480 cell lysate (20 µg/Lane)<br />Lane 2: MCF7 cell lysate (20 µg/Lane)<br />Lane 3: SK-Br-3 cell lysate (20 µg/Lane)<br />Lane 4: SiHa cell lysate (20 µg/Lane)<br />Lane 5: PC-3M cell lysate (20 µg/Lane)<br />Lane 6: HeLa cell lysate (20 µg/Lane)<br />Lane 7: Ramos cell lysate (20 µg/Lane)<br />Lane 8: Mouse testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 110 kDa<br />Observed band size: 110/50 kDa<br /><br />Exposure time: 30 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7106-90" style="font-weight: bold;text-decoration: underline;">ET7106-90</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cellular Apoptosis Susceptibility on different lysates with Rabbit anti-Cellular Apoptosis Susceptibility antibody (ET7106-90) at 1/1,000 dilution.

    Lane 1: SW480 cell lysate (20 µg/Lane)
    Lane 2: MCF7 cell lysate (20 µg/Lane)
    Lane 3: SK-Br-3 cell lysate (20 µg/Lane)
    Lane 4: SiHa cell lysate (20 µg/Lane)
    Lane 5: PC-3M cell lysate (20 µg/Lane)
    Lane 6: HeLa cell lysate (20 µg/Lane)
    Lane 7: Ramos cell lysate (20 µg/Lane)
    Lane 8: Mouse testis tissue lysate (40 µg/Lane)

    Predicted band size: 110 kDa
    Observed band size: 110/50 kDa

    Exposure time: 30 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-90) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • ICC staining Cellular Apoptosis Susceptibility in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Cellular Apoptosis Susceptibility in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Cellular Apoptosis Susceptibility in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Cellular Apoptosis Susceptibility in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Cellular Apoptosis Susceptibility in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Cellular Apoptosis Susceptibility in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cellular Apoptosis Susceptibility antibody (<a href="/products/ET7106-90" style="font-weight: bold;text-decoration: underline;">ET7106-90</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7106-90" style="font-weight: bold;text-decoration: underline;">ET7106-90</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cellular Apoptosis Susceptibility antibody (ET7106-90) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-90) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Cellular Apoptosis Susceptibility antibody (<a href="/products/ET7106-90" style="font-weight: bold;text-decoration: underline;">ET7106-90</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7106-90" style="font-weight: bold;text-decoration: underline;">ET7106-90</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Cellular Apoptosis Susceptibility antibody (ET7106-90) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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