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DEK Recombinant Rabbit Monoclonal Antibody [JB36-32]

Usd: 385

Specification

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Catalog# ET7107-99

DEK Recombinant Rabbit Monoclonal Antibody [JB36-32]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

DEK Recombinant Rabbit Monoclonal Antibody [JB36-32]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human DEK aa 31-220 / 375.

Species Reactivity

Human, Rat

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 43 kDa

Positive Control

HeLa cell lysate, A431 cell lysate, K-562 cell lysate, HEK-293 cell lysate, PC-12 cell lysate, A431.

Conjugation

unconjugated

Clone Number

JB36-32

RRID

AB_3070803

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:250

  • FC

  • 1:1,000

Target

Function

The mammalian nuclear phosphoprotein DEK is implicated in multiple cellular processes, including transcriptional regulation, mRNA processing, and chromatin remodeling, and is associated with a number of clinical autoimmune and neoplastic conditions. DEK, an abundant chromatin-associated protein, changes the topology of DNA in chromatin in vitro. Although first identified in a fusion with the CAN/Nup214 nucleoporin protein in a specific subtype of acute myelogenous leukemia, DEK is also an autoantigen in patients with Pauciarticular onset juvenile rheumatoid arthritis. Furthermore, the last 65 amino acids of DEK can partially reverse the mutation-prone phenotype of cells from patients with ataxia-telangiectasia. The human DEK gene maps to chromosome 6p22.3.

Background References

1. Hu H.G et al. The distribution of the DEK protein in mammalian chromatin. Biochem Biophys Res Commun 358:1008-1014 (2007).

2. Takata H et al. Proteome analysis of human nuclear insoluble fractions. Genes Cells 14:975-990 (2009).

Tissue Specificity

Ubiquitous. Expressed at relatively high levels.

Post-translational Modification

Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P35659 Human

SwissProt: Q6AXS3 Rat

Synonyms

D6S231E antibody

Dek antibody

DEK gene antibody

DEK oncogene antibody

DEK oncogene DNA binding antibody

DEK_HUMAN antibody

Protein DEK antibody

Images

  • Western blot analysis of DEK on different lysates with Rabbit anti-DEK antibody (<a href="/products/ET7107-99" style="font-weight: bold;text-decoration: underline;">ET7107-99</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A431 cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: HEK-293 cell lysate<br />Lane 5: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 43 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 40 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-99" style="font-weight: bold;text-decoration: underline;">ET7107-99</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DEK on different lysates with Rabbit anti-DEK antibody (ET7107-99) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A431 cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: HEK-293 cell lysate
    Lane 5: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 43 kDa
    Observed band size: 50 kDa

    Exposure time: 40 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-99) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of A431 cells labeling DEK with Rabbit anti-DEK antibody (<a href="/products/ET7107-99" style="font-weight: bold;text-decoration: underline;">ET7107-99</a>) at 1/250 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DEK antibody (<a href="/products/ET7107-99" style="font-weight: bold;text-decoration: underline;">ET7107-99</a>) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of A431 cells labeling DEK with Rabbit anti-DEK antibody (ET7107-99) at 1/250 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DEK antibody (ET7107-99) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of A431 cells labeling DEK.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET7107-99" style="font-weight: bold;text-decoration: underline;">ET7107-99</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A431 cells labeling DEK.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET7107-99, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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Citation